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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-2-14
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pubmed:abstractText |
The molecular basis for virus-induced disease in plants has been a long-standing mystery. Infection of Arabidopsis by Turnip mosaic virus (TuMV) induces a number of developmental defects in vegetative and reproductive organs. We found that these defects, many of which resemble those in miRNA-deficient dicer-like1 (dcl1) mutants, were due to the TuMV-encoded RNA-silencing suppressor, P1/HC-Pro. Suppression of RNA silencing is a counterdefensive mechanism that enables systemic infection by TuMV. The suppressor interfered with the activity of miR171 (also known as miRNA39), which directs cleavage of several mRNAs coding for Scarecrow-like transcription factors, by inhibiting miR171-guided nucleolytic function. Out of ten other mRNAs that were validated as miRNA-guided cleavage targets, eight accumulated to elevated levels in the presence of P1/HC-Pro. The basis for TuMV- and other virus-induced disease in plants may be explained, at least partly, by interference with miRNA-controlled developmental pathways that share components with the antiviral RNA-silencing pathway.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/HC-Pro protein, potyvirus,
http://linkedlifedata.com/resource/pubmed/chemical/MicroRNAs,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Plant,
http://linkedlifedata.com/resource/pubmed/chemical/SCR protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1534-5807
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
205-17
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12586064-Arabidopsis,
pubmed-meshheading:12586064-Arabidopsis Proteins,
pubmed-meshheading:12586064-Blotting, Northern,
pubmed-meshheading:12586064-Cysteine Endopeptidases,
pubmed-meshheading:12586064-DNA Primers,
pubmed-meshheading:12586064-Gene Expression Regulation, Developmental,
pubmed-meshheading:12586064-Gene Expression Regulation, Plant,
pubmed-meshheading:12586064-Immunoblotting,
pubmed-meshheading:12586064-MicroRNAs,
pubmed-meshheading:12586064-Microscopy, Electron, Scanning,
pubmed-meshheading:12586064-Microscopy, Polarization,
pubmed-meshheading:12586064-Mutagenesis, Site-Directed,
pubmed-meshheading:12586064-Plant Structures,
pubmed-meshheading:12586064-Plants, Genetically Modified,
pubmed-meshheading:12586064-Plasmids,
pubmed-meshheading:12586064-Polymerase Chain Reaction,
pubmed-meshheading:12586064-RNA, Messenger,
pubmed-meshheading:12586064-RNA, Plant,
pubmed-meshheading:12586064-RNA Interference,
pubmed-meshheading:12586064-Tymovirus,
pubmed-meshheading:12586064-Viral Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction.
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pubmed:affiliation |
Center for Gene Research and Biotechnology and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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