Linked Life Data

12583610

Source:http://linkedlifedata.com/resource/pubmed/id/12583610

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-2-13
pubmed:abstractText
Experimental autoimmune myocarditis is provoked by immunization with cardiac myosin. This animal model finally develops into dilated cardiomyopathy through repetitive myosin injections. To identify the myocardiogenic epitope, therefore, it is imperative not only to understand the mechanism of induction, but also to produce specific therapies, such as a blocking therapy to suppress the autoimmune process. Thus, we attempted to identify the myocarditogenic epitope using recombinant peptides. Beta-cardiac myosin heavy chain (CMHC) was amplified from rat mRNA by a reverse transcription polymerase chain reaction method. The PCR primers were designed to narrow the epitopic amino acid portion from each N-terminal to C-terminal site. These PCR products were cloned into an E. coli expression vector to produce fusion proteins consisting of a Histidine-tag and a myosin peptide. The segment of amplified CMHC including the epitopic amino acid sequence to provoke moderate myocarditis in vivo was reported previously. Each peptide solution was emulsified in an equal volume of complete Freund's adjuvant and given as an immunization to 7-week-old rats. On day 21 after immunization, the rats were sacrificed, and the fresh heart was observed pathologically. Through this immunization, we could restrict the myocardiogenic site. Lastly, this peptide was found to be located on residues from 1,124 to 1,153. Using ELISA, the antibodies against myocarditogenic peptides were easily identified. Whether or not the antibody productivity is linked to myocarditogenecity is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0918-2918
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Myocarditogenic epitopes and autoimmune myocarditis.
pubmed:affiliation
Department of Internal Medicine and Cardiology and Gene Genetics, Kitasato University School of Medicine, Sagamihara.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't