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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-2-19
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pubmed:databankReference |
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pubmed:abstractText |
Beta-arrestins bind to activated G protein-coupled receptor kinase-phosphorylated receptors, which leads to their desensitization with respect to G proteins, internalization via clathrin-coated pits, and signaling via a growing list of "scaffolded" pathways. To facilitate the discovery of novel adaptor and signaling roles of beta-arrestins, we have developed and validated a generally applicable interfering RNA approach for selectively suppressing beta-arrestins 1 or 2 expression by up to 95%. Beta-arrestin depletion in HEK293 cells leads to enhanced cAMP generation in response to beta(2)-adrenergic receptor stimulation, markedly reduced beta(2)-adrenergic receptor and angiotensin II receptor internalization and impaired activation of the MAP kinases ERK 1 and 2 by angiotensin II. This approach should allow discovery of novel signaling and regulatory roles for the beta-arrestins in many seven-membrane-spanning receptor systems.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-10097102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-10196135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-10352011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-10393547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-10725339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-10748214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-11090355,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-11171997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-11226259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-11248546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-11313899,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-11373684,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-11777902,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-11861753,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-12056542,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-12360232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-12370187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-1517224,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-2163110,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-4371263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-7507928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-8569717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-8702465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-8837779,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12582207-9759500
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
100
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1740-4
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12582207-Arrestins,
pubmed-meshheading:12582207-Base Sequence,
pubmed-meshheading:12582207-Blotting, Western,
pubmed-meshheading:12582207-Cell Line,
pubmed-meshheading:12582207-DNA Primers,
pubmed-meshheading:12582207-Endocytosis,
pubmed-meshheading:12582207-Enzyme Activation,
pubmed-meshheading:12582207-Humans,
pubmed-meshheading:12582207-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:12582207-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:12582207-Mitogen-Activated Protein Kinases,
pubmed-meshheading:12582207-Molecular Sequence Data,
pubmed-meshheading:12582207-Phosphorylation,
pubmed-meshheading:12582207-RNA, Small Interfering,
pubmed-meshheading:12582207-Signal Transduction
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pubmed:year |
2003
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pubmed:articleTitle |
Desensitization, internalization, and signaling functions of beta-arrestins demonstrated by RNA interference.
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pubmed:affiliation |
The Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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