Linked Life Data

12581795

Source:http://linkedlifedata.com/resource/pubmed/id/12581795

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2003-2-12
pubmed:abstractText
Calcium homeostasis, in terms of both cytosolic calcium concentration ([Ca(2+)](i)) and capacitative calcium entry, has been investigated in human skin and lung fibroblasts at different developmental and ageing stages by employing the Fura-2 based measurements. [Ca(2+)](i) in foetal lung or skin cells were nearly similar. However, significant changes were observed between foetal and adult fibroblasts and interestingly in opposite directions depending on the tissue of origin. In particular, in adult lung cells [Ca(2+)](i) was more than three-fold higher compared to adult skin fibroblasts, a difference which may contribute to tissue-specific functions. Capacitative Ca(2+) entry, i.e. the transient [Ca(2+)](i) increase induced by re-addition of extracellular calcium after depletion of thapsigargin-sensitive calcium stores, was found to exhibit the same pattern of differences during foetal-to-adult transition (it is two-fold higher in adult lung cells than in adult skin cells). At variance, we found capacitative Ca(2+) entry to be significantly attenuated during in vivo or in vitro ageing of fibroblasts; while minor alterations of [Ca(2+)](i) were observed. These findings indicate that capacitative calcium entry rather than [Ca(2+)](i), is mainly affected during the ageing process.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0531-5565
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
307-11
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Developmental and age-related alterations of calcium homeostasis in human fibroblasts.
pubmed:affiliation
Department of Animal and Human Physiology, School of Biology, Faculty of Sciences, University of Athens, 157 84 Panepistimiopolis, Athens, Greece. ppapaz@biol.uoa.gr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't