| pubmed-article:12576161 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12576161 | lifeskim:mentions | umls-concept:C0525033 | lld:lifeskim |
| pubmed-article:12576161 | lifeskim:mentions | umls-concept:C0014834 | lld:lifeskim |
| pubmed-article:12576161 | lifeskim:mentions | umls-concept:C0015733 | lld:lifeskim |
| pubmed-article:12576161 | lifeskim:mentions | umls-concept:C2347026 | lld:lifeskim |
| pubmed-article:12576161 | lifeskim:mentions | umls-concept:C0370003 | lld:lifeskim |
| pubmed-article:12576161 | lifeskim:mentions | umls-concept:C0080194 | lld:lifeskim |
| pubmed-article:12576161 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
| pubmed-article:12576161 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:12576161 | lifeskim:mentions | umls-concept:C0032520 | lld:lifeskim |
| pubmed-article:12576161 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:12576161 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12576161 | pubmed:dateCreated | 2003-2-10 | lld:pubmed |
| pubmed-article:12576161 | pubmed:abstractText | PCR was used to establish a specific detection system for the non-pathogenic Escherichia coli strain Nissle 1917 (DSM6601), which is used as a probiotic drug against intestinal disorders and diseases. Five PCR assays have been developed which are based on the chromosomally encoded major fimbrial subunit genes fimA (type 1 fimbriae) and focA (F1C fimbriae), and the two small cryptic plasmids pMUT1 and pMUT2. The assays were validated by testing a collection of 354 different pathogenic and non-pathogenic E. coli strains from various origins, including E. coli K-12, fecal and environmental as well as pathogenic extraintestinal and intestinal E. coli strains. The most specific results were obtained with primers based on DNA sequences from plasmid pMUT2. The plasmid-based PCR assays described can be used to detect E. coli strain Nissle 1917 in feces from patients without prior cultivation. | lld:pubmed |
| pubmed-article:12576161 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12576161 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12576161 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12576161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12576161 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12576161 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12576161 | pubmed:issn | 0923-2508 | lld:pubmed |
| pubmed-article:12576161 | pubmed:author | pubmed-author:KruisWolfgang... | lld:pubmed |
| pubmed-article:12576161 | pubmed:author | pubmed-author:HackerJörgJ | lld:pubmed |
| pubmed-article:12576161 | pubmed:author | pubmed-author:Blum-OehlerGa... | lld:pubmed |
| pubmed-article:12576161 | pubmed:author | pubmed-author:SchulzeJürgen... | lld:pubmed |
| pubmed-article:12576161 | pubmed:author | pubmed-author:SonnenbornUlr... | lld:pubmed |
| pubmed-article:12576161 | pubmed:author | pubmed-author:OswaldSibylle... | lld:pubmed |
| pubmed-article:12576161 | pubmed:author | pubmed-author:EiteljörgeKar... | lld:pubmed |
| pubmed-article:12576161 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12576161 | pubmed:volume | 154 | lld:pubmed |
| pubmed-article:12576161 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12576161 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12576161 | pubmed:pagination | 59-66 | lld:pubmed |
| pubmed-article:12576161 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12576161 | pubmed:meshHeading | pubmed-meshheading:12576161... | lld:pubmed |
| pubmed-article:12576161 | pubmed:meshHeading | pubmed-meshheading:12576161... | lld:pubmed |
| pubmed-article:12576161 | pubmed:meshHeading | pubmed-meshheading:12576161... | lld:pubmed |
| pubmed-article:12576161 | pubmed:meshHeading | pubmed-meshheading:12576161... | lld:pubmed |
| pubmed-article:12576161 | pubmed:meshHeading | pubmed-meshheading:12576161... | lld:pubmed |
| pubmed-article:12576161 | pubmed:meshHeading | pubmed-meshheading:12576161... | lld:pubmed |
| pubmed-article:12576161 | pubmed:meshHeading | pubmed-meshheading:12576161... | lld:pubmed |
| pubmed-article:12576161 | pubmed:articleTitle | Development of strain-specific PCR reactions for the detection of the probiotic Escherichia coli strain Nissle 1917 in fecal samples. | lld:pubmed |
| pubmed-article:12576161 | pubmed:affiliation | Institut für Molekulare Infektionsbiologie, Röntgenring 11, D-97070, Würzburg, Germany | lld:pubmed |
| pubmed-article:12576161 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12576161 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12576161 | lld:pubmed |
