Source:http://linkedlifedata.com/resource/pubmed/id/12574363
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0003320,
umls-concept:C0022702,
umls-concept:C0037791,
umls-concept:C0039194,
umls-concept:C0040558,
umls-concept:C0085358,
umls-concept:C0205263,
umls-concept:C0591833,
umls-concept:C1306673,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1704788,
umls-concept:C1706438,
umls-concept:C2698600
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| pubmed:issue |
4
|
| pubmed:dateCreated |
2003-2-7
|
| pubmed:abstractText |
Toxoplasma gondii forms different life stages, fast-replicating tachyzoites and slow-growing bradyzoites, in mammalian hosts. CD8 T cells are of crucial importance in toxoplasmosis, but it is unknown which parasite stage is recognized by CD8 T cells. To analyze stage-specific CD8 T cell responses, we generated various recombinant Toxoplasma gondii expressing the heterologous Ag beta-galactosidase (beta-gal) and studied whether 1) secreted or cytoplasmic Ags and 2) tachyzoites or bradyzoites, which persist intracerebrally, induce CD8 T cells. We monitored the frequencies and kinetics of beta-gal-specific CD8 T cells in infected mice by MHC class I tetramer staining. Upon oral infection of B6C (H-2(bxd)) mice, only beta-gal-secreting tachyzoites induced beta-gal-specific CD8 T cells. However, upon secondary infection of mice that had received a primary infection with tachyzoites secreting beta-gal, beta-gal-secreting tachyzoites and bradyzoites transiently increased the frequency of intracerebral beta-gal-specific CD8 T cells. Frequencies of splenic and cerebral beta-gal-specific CD8 T cells peaked at day 23 after infection, thereafter persisting at high levels in the brain but declining in the spleen. Splenic and cerebral beta-gal-specific CD8 T cells produced IFN-gamma and were cytolytic upon specific restimulation. Thus, compartmentalization and stage specificity of an Ag determine the induction of CD8 T cells in toxoplasmosis.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
170
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1949-57
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12574363-Animals,
pubmed-meshheading:12574363-Animals, Genetically Modified,
pubmed-meshheading:12574363-Antigens, Protozoan,
pubmed-meshheading:12574363-Brain,
pubmed-meshheading:12574363-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12574363-Cytotoxicity, Immunologic,
pubmed-meshheading:12574363-Epitopes, T-Lymphocyte,
pubmed-meshheading:12574363-Genetic Vectors,
pubmed-meshheading:12574363-Immunization, Secondary,
pubmed-meshheading:12574363-Kinetics,
pubmed-meshheading:12574363-Lymphocyte Activation,
pubmed-meshheading:12574363-Mice,
pubmed-meshheading:12574363-Mice, Inbred BALB C,
pubmed-meshheading:12574363-Mice, Inbred C57BL,
pubmed-meshheading:12574363-Organ Specificity,
pubmed-meshheading:12574363-Peptide Fragments,
pubmed-meshheading:12574363-Spleen,
pubmed-meshheading:12574363-Toxoplasma,
pubmed-meshheading:12574363-Toxoplasmosis, Animal,
pubmed-meshheading:12574363-beta-Galactosidase
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| pubmed:year |
2003
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| pubmed:articleTitle |
The induction and kinetics of antigen-specific CD8 T cells are defined by the stage specificity and compartmentalization of the antigen in murine toxoplasmosis.
|
| pubmed:affiliation |
Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Mannheim, Universität Heidelberg, Theodor-Kutzer-Ufer 1-3, D-68167 Mannheim, Germany.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|