Source:http://linkedlifedata.com/resource/pubmed/id/12574337
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2003-2-7
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| pubmed:abstractText |
The Qa-1(b)/Qdm tetramer binds to CD94/NKG2 receptors expressed at high levels on approximately 50% of murine NK cells. Although very few CD8 T cells from naive mice express CD94/NKG2 receptors, approximately 50% of CD8 T cells taken from mice undergoing a secondary response against Listeria monocytogenes (LM) are CD94(high) and bind the tetramer. Although CD94(int) NK cells do not bind the tetramer, CD94(int) CD8 T cells do, and this binding is dependent on the CD8 coreceptor. We found that the extent of apoptosis in CD8 T and NK cells was inversely related to the expression of CD94, with lower levels of apoptosis seen in CD94(high) cells after 1-3 days of culture. The difference in CD8 T cell survival was evident as early as 6 h after culture and persisted until nearly all the CD94(neg/int) cells were apoptotic by 48 h. In contrast, expression of inhibitory Ly-49A,G2,C/I molecules was associated with higher levels of apoptosis. Cross-linking CD94/NKG2 receptors on CD8 T cells from a mouse undergoing an LM infection further reduced the percentage of apoptotic cells on the CD94-expressing populations, while cross-linking Ly-49I had no effect on CD8 T cells expressing Ly-49I. Cross-linking CD3 on CD8 T cells from a mouse undergoing a secondary LM infection increases the extent of apoptosis, but this is prevented by cross-linking CD94/NKG2 receptors at the same time. Similar results were observed with NK cells in that the CD94(high) population displayed less apoptosis than CD94(int) cells after 1-3 days in culture. Therefore, the expression of CD94/NKG2 is correlated with a lower level of apoptosis and may play an important role in the maintenance of CD8 T and NK cells.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Ly,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Klrd1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/NK Cell Lectin-Like Receptor...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Mitogen,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, NK Cell Lectin-Like,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Natural Killer Cell
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
15
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| pubmed:volume |
170
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1737-45
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:12574337-Animals,
pubmed-meshheading:12574337-Antibodies, Monoclonal,
pubmed-meshheading:12574337-Antigens, CD,
pubmed-meshheading:12574337-Antigens, Ly,
pubmed-meshheading:12574337-Apoptosis,
pubmed-meshheading:12574337-CD8-Positive T-Lymphocytes,
pubmed-meshheading:12574337-Cell Survival,
pubmed-meshheading:12574337-Cells, Cultured,
pubmed-meshheading:12574337-Cross-Linking Reagents,
pubmed-meshheading:12574337-Killer Cells, Natural,
pubmed-meshheading:12574337-Lectins, C-Type,
pubmed-meshheading:12574337-Listeria monocytogenes,
pubmed-meshheading:12574337-Mice,
pubmed-meshheading:12574337-Mice, Inbred C57BL,
pubmed-meshheading:12574337-Mice, Transgenic,
pubmed-meshheading:12574337-NK Cell Lectin-Like Receptor Subfamily D,
pubmed-meshheading:12574337-Receptors, Immunologic,
pubmed-meshheading:12574337-Receptors, Mitogen,
pubmed-meshheading:12574337-Receptors, NK Cell Lectin-Like,
pubmed-meshheading:12574337-Receptors, Natural Killer Cell
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| pubmed:year |
2003
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| pubmed:articleTitle |
Preferential survival of CD8 T and NK cells expressing high levels of CD94.
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| pubmed:affiliation |
Center for Immunology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390-9093, USA.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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