Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-2-6
pubmed:abstractText
The aim of this study was to evaluate PCR and fluorescent in situ hybridization (FISH) techniques for detecting Arcobacter and Campylobacter strains in river water and wastewater samples. Both 16S and 23S rRNA sequence data were used to design specific primers and oligonucleotide probes for PCR and FISH analyses, respectively. In order to assess the suitability of the methods, the assays were performed on naturally and artificially contaminated samples and compared with the isolation of cells on selective media. The detection range of PCR and FISH assays varied between 1 cell/ml (after enrichment) to 10(3) cells/ml (without enrichment). According to our results, both rRNA-based techniques have the potential to be used as quick and sensitive methods for detection of campylobacters in environmental samples.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-10030029, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-11501676, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-1622752, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-1688842, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-1770362, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-2272939, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-3053218, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-7535888, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-7758934, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-7854248, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-8002483, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-8186099, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-8314996, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-8953731, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-9003624, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-9037670, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-9212435, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-9361442, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-9464415, http://linkedlifedata.com/resource/pubmed/commentcorrection/12571045-9750320
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
69
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1181-6
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Specific detection of Arcobacter and Campylobacter strains in water and sewage by PCR and fluorescent in situ hybridization.
pubmed:affiliation
Departamento de Biotecnología, Universidad Politécnica, 46022 Valencia, Spain.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't, Evaluation Studies