Linked Life Data

12570786

Source:http://linkedlifedata.com/resource/pubmed/id/12570786

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-2-6
pubmed:abstractText
Rapid Translation System (RTS) is a cell-free protein production system employing an enhanced Escherichia coli lysate to perform coupled in vitro transcription-translation reactions. A continuous supply of energy substrates, nucleotides and amino acids combined with the removal of by-products guarantees a high yield of protein production. The gene to express is either cloned into a plasmid vector or introduced as a PCR product amenable to automation. The main property of this alternative system to cellular expression systems is its open design allowing direct manipulation of the reaction conditions and applications that are impossible or difficult in cell-based systems. RTS offers new promising possibilities in the postgenomic era.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1389-2037
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-80
pubmed:dateRevised
2005-11-16
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Rapid translation system (RTS): a promising alternative for recombinant protein production.
pubmed:affiliation
Unité Repliement et Modélisation des Protéines, Institut Pasteur, CNRS - URA2185 28, rue du Dr. Roux 75724 Paris Cédex 15, France. jmbetton@pasteur.fr
pubmed:publicationType
Journal Article, Review