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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
2003-4-7
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pubmed:abstractText |
The Abl family of mammalian nonreceptor tyrosine kinases consists of c-Abl and Arg. Recent work has shown that c-Abl and Arg are activated in the cellular response to oxidative stress. The present studies demonstrate that reactive oxygen species (ROS) induce the formation of c-Abl and Arg heterodimers. The results show that the c-Abl SH3 domain binds directly to a proline-rich site (amino acids 567-576) in the Arg C-terminal region. Formation of c-Abl.Arg heterodimers also involves direct binding of the Arg Src homology 3 domain to the C-terminal region of c-Abl. The results further demonstrate that the interaction between c-Abl and Arg involves c-Abl-mediated phosphorylation of Arg. The functional significance of the c-Abl-Arg interaction is supported by the demonstration that both c-Abl and Arg are required for ROS-induced apoptosis. These findings indicate that ROS induce c-Abl.Arg heterodimers and that both c-Abl and Arg are necessary as effectors in the apoptotic response to oxidative stress.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
12961-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12569093-Amino Acid Sequence,
pubmed-meshheading:12569093-Animals,
pubmed-meshheading:12569093-Binding Sites,
pubmed-meshheading:12569093-Breast Neoplasms,
pubmed-meshheading:12569093-Cell Line,
pubmed-meshheading:12569093-Cells, Cultured,
pubmed-meshheading:12569093-Cloning, Molecular,
pubmed-meshheading:12569093-Female,
pubmed-meshheading:12569093-Fibroblasts,
pubmed-meshheading:12569093-Humans,
pubmed-meshheading:12569093-Kinetics,
pubmed-meshheading:12569093-Mice,
pubmed-meshheading:12569093-Oxidative Stress,
pubmed-meshheading:12569093-Peptide Fragments,
pubmed-meshheading:12569093-Protein-Tyrosine Kinases,
pubmed-meshheading:12569093-Proto-Oncogene Proteins c-abl,
pubmed-meshheading:12569093-Reactive Oxygen Species,
pubmed-meshheading:12569093-Recombinant Fusion Proteins,
pubmed-meshheading:12569093-Sequence Deletion,
pubmed-meshheading:12569093-Tumor Cells, Cultured,
pubmed-meshheading:12569093-src Homology Domains
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pubmed:year |
2003
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pubmed:articleTitle |
Functional interaction between the c-Abl and Arg protein-tyrosine kinases in the oxidative stress response.
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pubmed:affiliation |
Beijing Institute of Biotechnology, Beijing 100850, China.
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pubmed:publicationType |
Journal Article
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