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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-2-5
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pubmed:abstractText |
Thirty-five strains of Lactobacillus delbrueckii subsp. lactis and subsp. bulgaricus isolated from dairy products were typed by restriction fragment length polymorphism (RFLP) of protein-coding genes. The strains were analysed by RFLP of PCR amplified, infragenic fragments of the following housekeeping genes: beta-galactosidase, lactose permease, and proline dipeptidase. Sequencing of the variable regions of the 16S rDNA was then performed on a reduced number of strains. PCR-RFLP analysis evidenced wide strain heterogeneity. Strains were grouped into genotypes according to both subspecies assignment and infra-species genetic polymorphism. This polymorphism was related to the presence of microbial groups within subspecies populations. The low infra-species sequence polymorphism detected in the variable region of the 16S rRNA gene did not enable to group the strains with the same sensitivity reached by PCR-RFLP of protein-coding genes. PCR-RFLP of protein-coding genes applied to L. delbrueckii seems a promising tool to evaluate microbial diversity within bacterial subpopulations. Differences among bacterial subpopulations based upon molecular heterogeneity in protein-coding genes would enable to better understand the role of strains from different ecological niches.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/Dipeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/LacY protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase,
http://linkedlifedata.com/resource/pubmed/chemical/lactose permease,
http://linkedlifedata.com/resource/pubmed/chemical/proline dipeptidase
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0168-1605
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
25
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
163-72
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12568756-Bacterial Typing Techniques,
pubmed-meshheading:12568756-Cluster Analysis,
pubmed-meshheading:12568756-DNA, Bacterial,
pubmed-meshheading:12568756-DNA, Ribosomal,
pubmed-meshheading:12568756-Dairy Products,
pubmed-meshheading:12568756-Dipeptidases,
pubmed-meshheading:12568756-Escherichia coli Proteins,
pubmed-meshheading:12568756-Food Microbiology,
pubmed-meshheading:12568756-Genotype,
pubmed-meshheading:12568756-Lactobacillus,
pubmed-meshheading:12568756-Membrane Transport Proteins,
pubmed-meshheading:12568756-Monosaccharide Transport Proteins,
pubmed-meshheading:12568756-Phylogeny,
pubmed-meshheading:12568756-Polymerase Chain Reaction,
pubmed-meshheading:12568756-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:12568756-RNA, Ribosomal, 16S,
pubmed-meshheading:12568756-Restriction Mapping,
pubmed-meshheading:12568756-Symporters,
pubmed-meshheading:12568756-beta-Galactosidase
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pubmed:year |
2003
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pubmed:articleTitle |
Molecular typing of Lactobacillus delbrueckii of dairy origin by PCR-RFLP of protein-coding genes.
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pubmed:affiliation |
Department of Microbiology and Enzymology, Istituto Sperimentale Lattiero Caseario, Via A. Lombardo 11, 26900 Lodi, Italy. ggiraffa@ilclodi.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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