| pubmed-article:12566239 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12566239 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:12566239 | lifeskim:mentions | umls-concept:C1519658 | lld:lifeskim |
| pubmed-article:12566239 | lifeskim:mentions | umls-concept:C0023821 | lld:lifeskim |
| pubmed-article:12566239 | lifeskim:mentions | umls-concept:C2330716 | lld:lifeskim |
| pubmed-article:12566239 | lifeskim:mentions | umls-concept:C0008387 | lld:lifeskim |
| pubmed-article:12566239 | lifeskim:mentions | umls-concept:C0032979 | lld:lifeskim |
| pubmed-article:12566239 | lifeskim:mentions | umls-concept:C0243144 | lld:lifeskim |
| pubmed-article:12566239 | lifeskim:mentions | umls-concept:C1705313 | lld:lifeskim |
| pubmed-article:12566239 | lifeskim:mentions | umls-concept:C1515273 | lld:lifeskim |
| pubmed-article:12566239 | pubmed:issue | 2-3 | lld:pubmed |
| pubmed-article:12566239 | pubmed:dateCreated | 2003-2-4 | lld:pubmed |
| pubmed-article:12566239 | pubmed:abstractText | As villous trophoblast does represent the contact zone between foetal and maternal tissues, the present in vitro study was aimed at investigating cholesterol supply from human high density lipoprotein subclass 3 (HDL(3)) to trophoblast cells isolated from human first trimester and term placenta. Binding of (125)I-HDL(3) was specific and saturable with similar K(d)-values for first trimester (54 microg HDL(3)-protein/ml) and term villous trophoblast cells (29 microg HDL(3)-protein/ml). The cell-association of (125)I-HDL(3) was 3-fold higher for term trophoblast cells while the specific cell-association of [(3)H]cholesterol ester(CE)-labelled HDL(3) was higher for first trimester trophoblast preparations. As a consequence, first trimester trophoblast cells have a pronounced capacity for selective CE-uptake from HDL(3). Competition experiments with native and oxidized low-density lipoprotein as well as cAMP-mediated stimulation of cell-association of [(3)H]CE-HDL(3) in both trophoblast preparations suggested the scavenger receptor class B, type I (SR-BI) as a likely receptor mediating this pathway. SR-BI m RNA could be identified by RT-PCR and Northern blot experiments in both trophoblast preparations. Western blot analysis and immunocytochemistry revealed high expression of SR-BI in first trimester trophoblast. A polyclonal antiserum raised against murine SR-BI significantly decreased cell-association of [(3)H]CE-HDL(3) in trophoblast cells. We conclude that human first trimester and term trophoblast cells express SR-BI which could serve as an efficient route for supplying cholesterol esters from maternal lipoproteins to foetal tissues. | lld:pubmed |
| pubmed-article:12566239 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12566239 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12566239 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12566239 | pubmed:issn | 0143-4004 | lld:pubmed |
| pubmed-article:12566239 | pubmed:author | pubmed-author:SattlerWW | lld:pubmed |
| pubmed-article:12566239 | pubmed:author | pubmed-author:HammerAA | lld:pubmed |
| pubmed-article:12566239 | pubmed:author | pubmed-author:MalleEE | lld:pubmed |
| pubmed-article:12566239 | pubmed:author | pubmed-author:DesoyeGG | lld:pubmed |
| pubmed-article:12566239 | pubmed:author | pubmed-author:KozarskyK FKF | lld:pubmed |
| pubmed-article:12566239 | pubmed:author | pubmed-author:Levak-FrankSS | lld:pubmed |
| pubmed-article:12566239 | pubmed:author | pubmed-author:WadsackCC | lld:pubmed |
| pubmed-article:12566239 | pubmed:author | pubmed-author:HirschmuglBB | lld:pubmed |
| pubmed-article:12566239 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12566239 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:12566239 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12566239 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12566239 | pubmed:pagination | 131-43 | lld:pubmed |
| pubmed-article:12566239 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:12566239 | pubmed:articleTitle | Selective cholesteryl ester uptake from high density lipoprotein by human first trimester and term villous trophoblast cells. | lld:pubmed |
| pubmed-article:12566239 | pubmed:affiliation | Institute of Medical Biochemistry and Molecular Biology, Karl-Franzens University Graz, Austria. | lld:pubmed |
| pubmed-article:12566239 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12566239 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12566239 | lld:pubmed |
