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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2-3
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pubmed:dateCreated |
2003-2-4
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pubmed:abstractText |
As villous trophoblast does represent the contact zone between foetal and maternal tissues, the present in vitro study was aimed at investigating cholesterol supply from human high density lipoprotein subclass 3 (HDL(3)) to trophoblast cells isolated from human first trimester and term placenta. Binding of (125)I-HDL(3) was specific and saturable with similar K(d)-values for first trimester (54 microg HDL(3)-protein/ml) and term villous trophoblast cells (29 microg HDL(3)-protein/ml). The cell-association of (125)I-HDL(3) was 3-fold higher for term trophoblast cells while the specific cell-association of [(3)H]cholesterol ester(CE)-labelled HDL(3) was higher for first trimester trophoblast preparations. As a consequence, first trimester trophoblast cells have a pronounced capacity for selective CE-uptake from HDL(3). Competition experiments with native and oxidized low-density lipoprotein as well as cAMP-mediated stimulation of cell-association of [(3)H]CE-HDL(3) in both trophoblast preparations suggested the scavenger receptor class B, type I (SR-BI) as a likely receptor mediating this pathway. SR-BI m RNA could be identified by RT-PCR and Northern blot experiments in both trophoblast preparations. Western blot analysis and immunocytochemistry revealed high expression of SR-BI in first trimester trophoblast. A polyclonal antiserum raised against murine SR-BI significantly decreased cell-association of [(3)H]CE-HDL(3) in trophoblast cells. We conclude that human first trimester and term trophoblast cells express SR-BI which could serve as an efficient route for supplying cholesterol esters from maternal lipoproteins to foetal tissues.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD36,
http://linkedlifedata.com/resource/pubmed/chemical/Cholesterol Esters,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, HDL,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, HDL3,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Lipoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Scavenger,
http://linkedlifedata.com/resource/pubmed/chemical/SCARB1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Scarb1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Scavenger Receptors, Class B
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pubmed:status |
MEDLINE
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pubmed:issn |
0143-4004
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
131-43
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12566239-Adult,
pubmed-meshheading:12566239-Animals,
pubmed-meshheading:12566239-Antigens, CD36,
pubmed-meshheading:12566239-Binding, Competitive,
pubmed-meshheading:12566239-Blotting, Western,
pubmed-meshheading:12566239-CHO Cells,
pubmed-meshheading:12566239-Cell Adhesion,
pubmed-meshheading:12566239-Cholesterol Esters,
pubmed-meshheading:12566239-Chorionic Villi,
pubmed-meshheading:12566239-Cricetinae,
pubmed-meshheading:12566239-Cyclic AMP,
pubmed-meshheading:12566239-Female,
pubmed-meshheading:12566239-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:12566239-Humans,
pubmed-meshheading:12566239-Labor, Obstetric,
pubmed-meshheading:12566239-Lipoproteins, HDL,
pubmed-meshheading:12566239-Lipoproteins, HDL3,
pubmed-meshheading:12566239-Membrane Proteins,
pubmed-meshheading:12566239-Microscopy, Confocal,
pubmed-meshheading:12566239-Pregnancy,
pubmed-meshheading:12566239-Pregnancy Trimester, First,
pubmed-meshheading:12566239-RNA, Messenger,
pubmed-meshheading:12566239-Receptors, Immunologic,
pubmed-meshheading:12566239-Receptors, Lipoprotein,
pubmed-meshheading:12566239-Receptors, Scavenger,
pubmed-meshheading:12566239-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12566239-Scavenger Receptors, Class B,
pubmed-meshheading:12566239-Trophoblasts
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pubmed:articleTitle |
Selective cholesteryl ester uptake from high density lipoprotein by human first trimester and term villous trophoblast cells.
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pubmed:affiliation |
Institute of Medical Biochemistry and Molecular Biology, Karl-Franzens University Graz, Austria.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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