Source:http://linkedlifedata.com/resource/pubmed/id/12566006
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2003-2-4
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pubmed:abstractText |
Highly specific and sensitive procedures will be required to evaluate proteomes. Proximity ligation is a recently introduced mechanism for protein analysis. In this technique, the convergence of sets of protein-binding reagents on individual target molecules juxtaposes attached nucleic acid sequences. Through a ligation reaction a DNA reporter sequence is created, which can be amplified. The procedure thus encodes detected proteins as specific nucleic acid sequences in what may be viewed as a reverse translation reaction.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0958-1669
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
82-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12566006-Base Sequence,
pubmed-meshheading:12566006-DNA Probes,
pubmed-meshheading:12566006-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12566006-Macromolecular Substances,
pubmed-meshheading:12566006-Molecular Probe Techniques,
pubmed-meshheading:12566006-Nucleic Acid Amplification Techniques,
pubmed-meshheading:12566006-Oligonucleotides,
pubmed-meshheading:12566006-Protein Binding,
pubmed-meshheading:12566006-Proteins,
pubmed-meshheading:12566006-Sequence Analysis, Protein
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pubmed:year |
2003
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pubmed:articleTitle |
A sense of closeness: protein detection by proximity ligation.
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pubmed:affiliation |
The Beijer Laboratory, Department of Genetics and Pathology, Rudbeck Laboratory, Se-751 85 Uppsala, Sweden.
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pubmed:publicationType |
Journal Article,
Review,
Research Support, Non-U.S. Gov't
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