|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2003-2-3
|
|
pubmed:abstractText |
We studied the genetic relatedness of 47 Portuguese penicillin-resistant 9V and 23F Streptococcus pneumoniae of clinical origin, using pulsed-field gel electrophoresis (PFGE) and restriction fragment length polymorphism analysis. PFGE fingerprinting showed that 24 isolates of serotype 9V and 23 isolates of serotype 23F were variants of the Spain(9V)-3 and Spain(23F)-1 clones, respectively. Fingerprinting of pbp1a, pbp2b and pbp2x genes showed that all the penicillin-resistant clones gave similar HinfI restriction patterns. In this study, serotypes 9V and 23F have different clonal origins and identical PBP genotypes, suggesting a horizontal transfer of resistance. Visual and computer-assisted analysis of PFGE patterns correlated well (r = 0.983).
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0305-7453
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
51
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
409-14
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:12562712-Aminoacyltransferases,
pubmed-meshheading:12562712-Bacterial Proteins,
pubmed-meshheading:12562712-Carrier Proteins,
pubmed-meshheading:12562712-Cloning, Molecular,
pubmed-meshheading:12562712-Electrophoresis, Gel, Pulsed-Field,
pubmed-meshheading:12562712-Genotype,
pubmed-meshheading:12562712-Hexosyltransferases,
pubmed-meshheading:12562712-Multigene Family,
pubmed-meshheading:12562712-Muramoylpentapeptide Carboxypeptidase,
pubmed-meshheading:12562712-Penicillin Resistance,
pubmed-meshheading:12562712-Penicillin-Binding Proteins,
pubmed-meshheading:12562712-Peptide Mapping,
pubmed-meshheading:12562712-Peptidyl Transferases,
pubmed-meshheading:12562712-Pneumococcal Infections,
pubmed-meshheading:12562712-Portugal,
pubmed-meshheading:12562712-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12562712-Spain,
pubmed-meshheading:12562712-Streptococcus pneumoniae
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Two major Spanish clones of penicillin-resistant Streptococcus pneumoniae in Portuguese isolates of clinical origin.
|
|
pubmed:affiliation |
Antibiotic Resistance Unit, Centre of Bacteriology, National Institute of Health Dr. Ricardo Jorge, Av. Padre Cruz, 1649-016 Lisbon, Portugal. manuela.canica@insa.min.saude.pt
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
