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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2003-2-3
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pubmed:databankReference |
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pubmed:abstractText |
By using the mouse zinc finger protein gene ZF-12 genomic DNA fragment, pSSC-TV-10.5 was designed and constructed as a replacement vector. Structure of pSSC-TV-10.5 was identified by restrictive digestion analysis and partly sequencing. Then linearized vector was electroporated into ES cells, and transfected cells were screened by G418 and GANC selection. Among 508 G418r/GANCr colonies, 4 were proved to have taken place the homologous recombination of ZF-12 by PCR and southern blotting analysis. This study lays the foundations of preparing mouse models of ZF-12+/- or ZF-12-/-.
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pubmed:language |
chi
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0379-4172
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
860-4
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12561468-Animals,
pubmed-meshheading:12561468-Blotting, Southern,
pubmed-meshheading:12561468-Carrier Proteins,
pubmed-meshheading:12561468-Cell Division,
pubmed-meshheading:12561468-Cell Survival,
pubmed-meshheading:12561468-DNA,
pubmed-meshheading:12561468-Electroporation,
pubmed-meshheading:12561468-Embryo, Mammalian,
pubmed-meshheading:12561468-Ganciclovir,
pubmed-meshheading:12561468-Genetic Vectors,
pubmed-meshheading:12561468-Genotype,
pubmed-meshheading:12561468-Gentamicins,
pubmed-meshheading:12561468-Mice,
pubmed-meshheading:12561468-Mice, Inbred Strains,
pubmed-meshheading:12561468-Molecular Sequence Data,
pubmed-meshheading:12561468-Sequence Analysis, DNA,
pubmed-meshheading:12561468-Transfection,
pubmed-meshheading:12561468-Zinc Fingers
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pubmed:year |
2002
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pubmed:articleTitle |
[Establishment of mouse ZF-12+/- embryonic stem cells].
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pubmed:affiliation |
Department of Medical Genetics, Second Military University, Shanghai 200433, China.
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pubmed:publicationType |
Journal Article,
English Abstract,
Research Support, Non-U.S. Gov't
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