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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
Pt 2
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pubmed:dateCreated |
2003-1-31
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129732,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129733,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129734,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129735,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129736,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129737,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129738,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129739,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129740,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129741,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129742,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129743,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129744,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129745,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129746,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129747,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129748,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129749,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129750,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129751,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/AY129752
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pubmed:abstractText |
Viruses in the Phlebovirus genus of the family Bunyaviridae cause clinical syndromes ranging from a short, self-limiting febrile illness to fatal haemorrhagic fever. The genus currently consists of 68 antigenically distinct virus serotypes, most of which have not been genetically characterized. RT-PCR with four 'cocktail' primers was performed to amplify a region of the M segment of the genome of 24 phleboviruses included in the sandfly fever Naples, sandfly fever Sicilian and Punta Toro serocomplexes. Partial M segment sequences were successfully obtained and phylogenetic analysis was performed. The three resultant genotypic lineages were consistent with serological data. The sequence divergences were 27.6 % (nucleotide) and 25.7 % (amino acid) within the Sicilian serocomplex, 33.7 % (nucleotide) and 34.4 % (amino acid) within the Naples serocomplex and 35.6 % (nucleotide) and 37.5 % (amino acid) within the Punta Toro serocomplex. Overall, the diversities among viruses of Sicilian, Naples and Punta Toro serocomplexes were 48.2 % and 57.6 % at the nucleotide and amino acid levels, respectively. This high genetic divergence may explain the difficulties in designing a consensus primer pair for the amplification of all the phleboviruses using RT-PCR. It also suggests that infection with one genotype may not completely immunize against infection with all other genotypes in a given serocomplex. These findings have implications for potential vaccine development and may help explain clinical reports of multiple episodes of sandfly fever in the same individual.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1317
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
465-73
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:12560581-Amino Acid Sequence,
pubmed-meshheading:12560581-Animals,
pubmed-meshheading:12560581-Base Sequence,
pubmed-meshheading:12560581-Cricetinae,
pubmed-meshheading:12560581-DNA Primers,
pubmed-meshheading:12560581-Glycoproteins,
pubmed-meshheading:12560581-Humans,
pubmed-meshheading:12560581-Molecular Sequence Data,
pubmed-meshheading:12560581-Phlebotomus Fever,
pubmed-meshheading:12560581-Phlebovirus,
pubmed-meshheading:12560581-Phylogeny,
pubmed-meshheading:12560581-RNA, Viral,
pubmed-meshheading:12560581-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12560581-Sequence Alignment,
pubmed-meshheading:12560581-Viral Nonstructural Proteins,
pubmed-meshheading:12560581-Viral Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
Phylogenetic relationships among members of the genus Phlebovirus (Bunyaviridae) based on partial M segment sequence analyses.
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pubmed:affiliation |
Department of Pathology and Center for Tropical Diseases, University of Texas Medical Branch, Galveston 77555-0588, USA.
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pubmed:publicationType |
Journal Article
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