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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2003-1-31
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pubmed:abstractText |
We utilized the heat-sensitive mutant strain (Ts932), bearing a mutation at position 61 in the mitochondrial tRNA(Asp) gene, to identify nuclear genes involved in tRNA biogenesis; this mutant is defective in 3'-end processing and consequently in the production of mature mitochondrial tRNA(Asp). We transformed this strain with a yeast nuclear library and we isolated among other suppressors, an unknown, non-essential gene (called SMM1, corresponding to open reading frame YNR015w), which restored the growth on glycerol and a normal amount of processed tRNA(Asp) in the mutant. The gene contains a domain highly conserved in evolution from bacteria to human and its product has been recently shown to have dihydrouridine synthase activity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
303
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
63-8
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:12559567-Amino Acid Sequence,
pubmed-meshheading:12559567-Cell Nucleus,
pubmed-meshheading:12559567-Conserved Sequence,
pubmed-meshheading:12559567-DNA, Mitochondrial,
pubmed-meshheading:12559567-Evolution, Molecular,
pubmed-meshheading:12559567-Gene Expression Regulation, Fungal,
pubmed-meshheading:12559567-Genetic Complementation Test,
pubmed-meshheading:12559567-Mitochondria,
pubmed-meshheading:12559567-Molecular Sequence Data,
pubmed-meshheading:12559567-Mutation,
pubmed-meshheading:12559567-Nuclear Proteins,
pubmed-meshheading:12559567-Oxidoreductases,
pubmed-meshheading:12559567-RNA, Transfer, Asp,
pubmed-meshheading:12559567-Saccharomyces cerevisiae,
pubmed-meshheading:12559567-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:12559567-Sequence Homology, Amino Acid,
pubmed-meshheading:12559567-Temperature
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pubmed:year |
2003
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pubmed:articleTitle |
Nucleo-mitochondrial interactions in Saccharomyces cerevisiae: characterization of a nuclear gene suppressing a defect in mitochondrial tRNA(Asp) processing.
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pubmed:affiliation |
Department of Cell and Developmental Biology, Pasteur Institute - Cenci Bolognetti Foundation, University of Rome I, Piazzale Aldo Moro 5, Italy. teresa.rinaldi@uniroma1.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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