Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1975-11-6
pubmed:abstractText
Proteoglycans were extracted from bovine corneal stroma with 4M-guanidinum chloride, purified by DEAE-dellulose chromatography (Antonopoulos et al., 1974) and fractionated by precipitation with ethanol into three fractions of approximately equal weight. One of these fractions consisted of a proteoglycan that contained keratan sulphate as the only glycosaminoglycan. In the othertwo fractions proteoglycans that contained chondroitin sulphate, dermatan sulphate and keratan sulphate were present. Proteoglycans which had a more than tenfold excess of galactosaminoglycans over keratan sulphate could be obtianed by further subfractionation. The gel-chromatographic patterns of the glucosaminoglycans before and after digestion with chondroitinase AC differed for the fractions. The individual chondroitin sulphate chains seemed to be larger in cornea than in cartilage. Oligosaccharides, possibly covalently linked to the protein core of the proteoglycans, could be isolated from all fractions. The corneal proteoglycans were shown to have higher protein contents and to be of smaller molecular size than cartilage proteoglycans.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-13129238, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-13221568, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-13764036, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-13861728, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-13971270, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-14156071, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-14284693, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-14907713, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4228417, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4229556, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4232067, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4233217, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4237578, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4251467, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4263792, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4265839, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4266948, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4277351, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4280264, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4339719, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-4624705, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-5411426, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-5647268, http://linkedlifedata.com/resource/pubmed/commentcorrection/125581-6068017
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
491-500
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1975
pubmed:articleTitle
Fractionation of proteoglycans from bovine corneal stroma.
pubmed:publicationType
Journal Article