Linked Life Data

12557347

Source:http://linkedlifedata.com/resource/pubmed/id/12557347

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-1-31
pubmed:abstractText
An EcoRI DNA fragment containing the plasmid replicon, named ori165, was cloned from the plasmids of B. thuringiensis subsp. tenebrionis strains YBT-1765. The replicon ori165 was the first replicon cloned from this subspecie. Its size is approximately 11 kb. The replicon was minimized to an 8 kb fragment by deletion analysis. Southern hybridization exhibited that ori165 was coming from the plasmid detected with the highest molecular weight. A shuttle vector pBMB6071 based on ori165 was constructed. The stabilities of pBMB6071 in different B. thuringiensis host had great discrepancy. In strain 4Q7, a crystal-negative derivative of B. thuringiensis subsp. isrealensis, the stability was 100%. ori165 was compatible with ori1030 and ori2062 in strain BMB171, a crystal-negative derivative of B. thuringiensis subsp. kurstaki.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0001-6209
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
[The cloning of plasmid replicon ori165 from Bacillus thuringiensis subsp. thenebrionis].
pubmed:affiliation
College of Life Science and Technology, Huazhong Agricultural University, Key Laboratory of Agricultural Microbiology, Ministry of Agriculture, Wuhan 430070, China.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't