| pubmed-article:12554059 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12554059 | lifeskim:mentions | umls-concept:C0220847 | lld:lifeskim |
| pubmed-article:12554059 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:12554059 | lifeskim:mentions | umls-concept:C0001473 | lld:lifeskim |
| pubmed-article:12554059 | lifeskim:mentions | umls-concept:C0920283 | lld:lifeskim |
| pubmed-article:12554059 | lifeskim:mentions | umls-concept:C1860991 | lld:lifeskim |
| pubmed-article:12554059 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:12554059 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
| pubmed-article:12554059 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
| pubmed-article:12554059 | lifeskim:mentions | umls-concept:C1882932 | lld:lifeskim |
| pubmed-article:12554059 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
| pubmed-article:12554059 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12554059 | pubmed:dateCreated | 2003-1-29 | lld:pubmed |
| pubmed-article:12554059 | pubmed:abstractText | It has been shown that the Hepatitis C virus nonstructural NS3 protein possesses at least two enzymatic domains: a serine-protease domain and an adenosine triphosphatase (ATPase)/helicase domain. In this report, a truncated fragment of NS3 (26 kDa), representing main epitopes from the (ATPase)/helicase domain, has been expressed in Escherichia coli. The recombinant protein was purified by Ion Metal Affinity Chromatography (IMAC) with more than 90% purity. The recognition of B-cell linear epitopes in the NS3 protein was evaluated by immunoblot. The recombinant NS3 protein was reduced and carboxymethylated, and the recognition of either conformational and/or linear B-cell determinants was evaluated by ELISA. The inclusion of the recombinant NS3 protein in a third-generation diagnostic system UltraMicroELISA (UMELISA) allowed an increase in the sensitivity, due to the detection of a new variety of false-negative sera in blood donor test samples. | lld:pubmed |
| pubmed-article:12554059 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12554059 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12554059 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12554059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12554059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12554059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12554059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12554059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12554059 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12554059 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12554059 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:12554059 | pubmed:issn | 0009-9120 | lld:pubmed |
| pubmed-article:12554059 | pubmed:author | pubmed-author:RiveraJ MJM | lld:pubmed |
| pubmed-article:12554059 | pubmed:author | pubmed-author:RocaJJ | lld:pubmed |
| pubmed-article:12554059 | pubmed:author | pubmed-author:CaballeroAA | lld:pubmed |
| pubmed-article:12554059 | pubmed:author | pubmed-author:PonceMM | lld:pubmed |
| pubmed-article:12554059 | pubmed:author | pubmed-author:RodríguezDD | lld:pubmed |
| pubmed-article:12554059 | pubmed:author | pubmed-author:MusacchioAA | lld:pubmed |
| pubmed-article:12554059 | pubmed:author | pubmed-author:NarciandiR... | lld:pubmed |
| pubmed-article:12554059 | pubmed:author | pubmed-author:PentónNN | lld:pubmed |
| pubmed-article:12554059 | pubmed:author | pubmed-author:TalloY IYI | lld:pubmed |
| pubmed-article:12554059 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12554059 | pubmed:volume | 36 | lld:pubmed |
| pubmed-article:12554059 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12554059 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12554059 | pubmed:pagination | 41-9 | lld:pubmed |
| pubmed-article:12554059 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:meshHeading | pubmed-meshheading:12554059... | lld:pubmed |
| pubmed-article:12554059 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12554059 | pubmed:articleTitle | Antigenicity of a recombinant NS3 protein representative of ATPase/helicase domain from hepatitis C virus. | lld:pubmed |
| pubmed-article:12554059 | pubmed:affiliation | Diagnostics Production Plant, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana 10600, Cuba. | lld:pubmed |
| pubmed-article:12554059 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:12554059 | lld:pubmed |
