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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-1-29
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pubmed:abstractText |
It has been shown that the Hepatitis C virus nonstructural NS3 protein possesses at least two enzymatic domains: a serine-protease domain and an adenosine triphosphatase (ATPase)/helicase domain. In this report, a truncated fragment of NS3 (26 kDa), representing main epitopes from the (ATPase)/helicase domain, has been expressed in Escherichia coli. The recombinant protein was purified by Ion Metal Affinity Chromatography (IMAC) with more than 90% purity. The recognition of B-cell linear epitopes in the NS3 protein was evaluated by immunoblot. The recombinant NS3 protein was reduced and carboxymethylated, and the recognition of either conformational and/or linear B-cell determinants was evaluated by ELISA. The inclusion of the recombinant NS3 protein in a third-generation diagnostic system UltraMicroELISA (UMELISA) allowed an increase in the sensitivity, due to the detection of a new variety of false-negative sera in blood donor test samples.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0009-9120
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
41-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12554059-Adenosine Triphosphatases,
pubmed-meshheading:12554059-Chromatography, Affinity,
pubmed-meshheading:12554059-Cloning, Molecular,
pubmed-meshheading:12554059-DNA Helicases,
pubmed-meshheading:12554059-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12554059-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12554059-Epitopes,
pubmed-meshheading:12554059-Escherichia coli,
pubmed-meshheading:12554059-Fermentation,
pubmed-meshheading:12554059-Immunoblotting,
pubmed-meshheading:12554059-Models, Genetic,
pubmed-meshheading:12554059-Plasmids,
pubmed-meshheading:12554059-Protein Structure, Tertiary,
pubmed-meshheading:12554059-Recombinant Proteins,
pubmed-meshheading:12554059-Temperature,
pubmed-meshheading:12554059-Viral Nonstructural Proteins
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pubmed:year |
2003
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pubmed:articleTitle |
Antigenicity of a recombinant NS3 protein representative of ATPase/helicase domain from hepatitis C virus.
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pubmed:affiliation |
Diagnostics Production Plant, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, Havana 10600, Cuba.
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pubmed:publicationType |
Journal Article
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