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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-1-28
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pubmed:abstractText |
We established a quantitative real-time RT-PCR assay for the detection of chimeric BCR-ABL transcripts in archival formalin-fixed bone marrow trephines, both acrylate-embedded and paraffin-embedded. This new methodology enables determination of transcript levels in direct comparison to histopathological findings and therapeutic interventions during the time course of the disease in a retrospective and a prospective manner. We found an excellent correlation between the quantitative molecular data and the morphological evaluation as well as the clinical outcome for a cohort of chronic myeloid leukemia patients (n = 10). To the best of our knowledge, this is the first study demonstrating the feasibility of large-scale quantitative expression analysis in archival bone marrow trephines for monitoring molecular markers over several years or even decades.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-10482989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-10576511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-10583264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-10797300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-10865964,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-10965782,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-10985361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-11238091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-11255271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-11376866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-11476553,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-11535500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-11846608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-11846609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-1280814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-4126434,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-6580527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12552081-9894600
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1525-1578
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
54-60
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12552081-Base Sequence,
pubmed-meshheading:12552081-Bone Marrow,
pubmed-meshheading:12552081-Cohort Studies,
pubmed-meshheading:12552081-DNA Primers,
pubmed-meshheading:12552081-Genes, abl,
pubmed-meshheading:12552081-HL-60 Cells,
pubmed-meshheading:12552081-Humans,
pubmed-meshheading:12552081-K562 Cells,
pubmed-meshheading:12552081-Leukemia, Myelogenous, Chronic, BCR-ABL Positive,
pubmed-meshheading:12552081-Molecular Diagnostic Techniques,
pubmed-meshheading:12552081-Prognosis,
pubmed-meshheading:12552081-RNA, Neoplasm,
pubmed-meshheading:12552081-Retrospective Studies,
pubmed-meshheading:12552081-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:12552081-Sensitivity and Specificity,
pubmed-meshheading:12552081-Tissue Embedding,
pubmed-meshheading:12552081-Transcription, Genetic,
pubmed-meshheading:12552081-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
Quantitative intra-individual monitoring of BCR-ABL transcript levels in archival bone marrow trephines of patients with chronic myeloid leukemia.
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pubmed:affiliation |
Institute of Pathology, Medizinische Hochschule Hannover, Hannover, Germany. Bock.Oliver@MH-Hannover.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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