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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-1-28
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pubmed:abstractText |
HIV-1 Tat is required for the expression of the viral genome. Tat binds to an RNA stem-loop and mediates the recruitment of human coactivators to facilitate HIV-1 transcription. The coactivator and acetyltransferase CREB binding protein (CBP), and the paralog p300, are recruited to the HIV-1 promoter by Tat. Here we identify the interacting domains of Tat and CBP. Circular dichroism and pulldown assays show that full-length Tat binds to the KIX domain of CBP, but not to the C/H1 or CR2 domains. Circular dichroism and NMR studies of Tat deletion mutants localize the KIX-binding domain of Tat to the N-terminal 24 residues of Tat. Transient cotransfections demonstrate that exogenous KIX behaves as a dominant negative to Tat-mediated transcription in human T-cells, suggesting that Tat and KIX interact in vivo. These findings indicate that Tat targets the KIX domain of CBP and provide insight into the molecular interactions involved in regulating HIV-1 gene expression.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CREB-Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/CREBBP protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Crebbp protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/E1A-Associated p300 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Ep300 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tat,
http://linkedlifedata.com/resource/pubmed/chemical/KIF22 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Kinesin,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/tat Gene Products, Human...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-2960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
910-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12549909-Amino Acid Sequence,
pubmed-meshheading:12549909-Animals,
pubmed-meshheading:12549909-CREB-Binding Protein,
pubmed-meshheading:12549909-Circular Dichroism,
pubmed-meshheading:12549909-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:12549909-DNA-Binding Proteins,
pubmed-meshheading:12549909-E1A-Associated p300 Protein,
pubmed-meshheading:12549909-Gene Products, tat,
pubmed-meshheading:12549909-HIV-1,
pubmed-meshheading:12549909-Humans,
pubmed-meshheading:12549909-Jurkat Cells,
pubmed-meshheading:12549909-Kinesin,
pubmed-meshheading:12549909-Mice,
pubmed-meshheading:12549909-Molecular Sequence Data,
pubmed-meshheading:12549909-Nuclear Proteins,
pubmed-meshheading:12549909-Protein Binding,
pubmed-meshheading:12549909-Protein Folding,
pubmed-meshheading:12549909-Protein Structure, Secondary,
pubmed-meshheading:12549909-Protein Structure, Tertiary,
pubmed-meshheading:12549909-Recombinant Fusion Proteins,
pubmed-meshheading:12549909-T-Lymphocytes,
pubmed-meshheading:12549909-Trans-Activators,
pubmed-meshheading:12549909-Transcriptional Activation,
pubmed-meshheading:12549909-tat Gene Products, Human Immunodeficiency Virus
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pubmed:year |
2003
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pubmed:articleTitle |
Molecular recognition of the human coactivator CBP by the HIV-1 transcriptional activator Tat.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, Department of Chemistry, Colorado State University, Fort Collins, Colorado 80523-1870, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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