|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2003-1-24
|
|
pubmed:abstractText |
Two rainbow trout (Oncorhynchus mykiss) genes for interferon regulatory factors (IRF) 1 and 2 have been cloned and sequenced. The IRF-1 cDNA contains an open reading frame (ORF) of 996 nucleotides that translates into a 331 amino-acid putative peptide, with a 5' untranslated region (UTR) of 145bp and a 3' UTR of 481bp. The IRF-2 cDNA contains a 1035bp ORF that translates into a 344 amino-acid putative peptide, with a 5' UTR of 146bp and a 3' UTR of 925bp. In vivo, IRF-1 and IRF-2 are constitutively expressed in head kidney, gill and spleen but not liver. Both genes were induced in all the tissues examined. IRF-1 but not IRF-2 expression was significantly increased at the site of injection 1 week after DNA vaccination against viral haemorrhagic septicaemia virus. In vitro, IRF-1 and IRF-2 transcripts are present in unstimulated rainbow trout gonad cells and are up-regulated by poly I/C.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0145-305X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
27
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
111-26
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:12543125-Amino Acid Sequence,
pubmed-meshheading:12543125-Animals,
pubmed-meshheading:12543125-Base Sequence,
pubmed-meshheading:12543125-Cell Line,
pubmed-meshheading:12543125-Cloning, Molecular,
pubmed-meshheading:12543125-DNA-Binding Proteins,
pubmed-meshheading:12543125-Gene Expression,
pubmed-meshheading:12543125-Interferon Regulatory Factor-1,
pubmed-meshheading:12543125-Interferon Regulatory Factor-2,
pubmed-meshheading:12543125-Molecular Sequence Data,
pubmed-meshheading:12543125-Novirhabdovirus,
pubmed-meshheading:12543125-Oncorhynchus mykiss,
pubmed-meshheading:12543125-Phosphoproteins,
pubmed-meshheading:12543125-Repressor Proteins,
pubmed-meshheading:12543125-Transcription Factors,
pubmed-meshheading:12543125-Vaccines, DNA,
pubmed-meshheading:12543125-Viral Vaccines
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Cloning and expression analysis of rainbow trout Oncorhynchus mykiss interferon regulatory factor 1 and 2 (IRF-1 and IRF-2).
|
|
pubmed:affiliation |
Department of Zoology, Scottish Fish Immunology Research Centre, University of Aberdeen, Tillydrone Avenue, Aberdeen, AB24 2TZ, UK.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
