Linked Life Data

12542861

Source:http://linkedlifedata.com/resource/pubmed/id/12542861

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2003-2-7
pubmed:abstractText
This study was designed to examine the effects of bone marrow stromal cells (MSCs) cultured in vitro with or without neurotrophic factors transplanted into adult male Wistar rats after traumatic brain injury (TBI). MSCs harvested from donor Wistar rats were cultured with either the culture medium containing brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) or the same culture media without these factors. Control and experimental animals were then traumatized by a controlled cortical impact. One day after the impact, either the placebo or the washed MSCs (1 x 10(6)) cultured with or without NGF and BDNF were transplanted adjacent to the site of injury. In addition, a nontreated group of rats was employed. Motor function of the animals was evaluated by the Rotarod test both before and after the injury. All animals were sacrificed 8 days after TBI, and the brain sections were stained by H&E as well as for immunohistochemistry. MSCs survived and migrated toward the injury site. The group treated with MSCs cultured with BDNF and NGF had a significantly higher number of engrafted cells than the group treated with MSCs cultured without BDNF and NGF (6.3 x 10(4) +/- 4250 compared to 4.1 x 10(4) +/- 3684; p < 0.05). In both groups, some transplanted MSCs showed positive staining for astrocytic (GFAP) and neuronal markers (Neu N and MAP-2). The groups treated with MSCs had better motor function than the groups receiving no treatment or receiving the placebo (PBS; p < 0.05); however, the improvement reached statistical significance only in the group treated with MSCs cultured with neurotrophic factors. These data suggest that more robust motor function described in rats subjected to TBI and treated with intracerebral transplantation of MSCs was achieved by the use of MSCs cultured with neurotrophic factors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0897-7151
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1609-17
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12542861-Animals, pubmed-meshheading:12542861-Biological Markers, pubmed-meshheading:12542861-Bone Marrow Transplantation, pubmed-meshheading:12542861-Brain Injuries, pubmed-meshheading:12542861-Brain-Derived Neurotrophic Factor, pubmed-meshheading:12542861-Cell Transplantation, pubmed-meshheading:12542861-Cells, Cultured, pubmed-meshheading:12542861-Disease Models, Animal, pubmed-meshheading:12542861-Glial Fibrillary Acidic Protein, pubmed-meshheading:12542861-Immunohistochemistry, pubmed-meshheading:12542861-Male, pubmed-meshheading:12542861-Microtubule-Associated Proteins, pubmed-meshheading:12542861-Nerve Growth Factors, pubmed-meshheading:12542861-Rats, pubmed-meshheading:12542861-Rats, Wistar, pubmed-meshheading:12542861-Recovery of Function, pubmed-meshheading:12542861-Stromal Cells, pubmed-meshheading:12542861-Treatment Outcome
pubmed:year
2002
pubmed:articleTitle
Intracerebral transplantation of marrow stromal cells cultured with neurotrophic factors promotes functional recovery in adult rats subjected to traumatic brain injury.
pubmed:affiliation
Department of Neurosurgery, Henry Ford Hospital, Detroit, Michigan 48202, USA. nsaam@neuro.hfh.edu
pubmed:publicationType
Journal Article, Comparative Study