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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2003-1-24
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pubmed:abstractText |
We have used differential display to profile and compare the mRNAs expressed in the hippocampus of freely moving animals after the induction of long-term potentiation (LTP) at the perforant path-dentate gyrus synapse with control rats receiving low-frequency stimulation. We have combined this with in situ hybridization and have identified A-kinase anchoring protein of 150 kDa (AKAP-150) as a gene selectively up-regulated during the maintenance phase of LTP. AKAP-150 mRNA has a biphasic modulation in the dentate gyrus following the induction of LTP. The expression of AKAP-150 was 29% lower than stimulated controls 1 h after the induction of LTP. Its expression was enhanced 3 (50%), 6 (239%) and 12 h (210%) after induction, returning to control levels by 24 h postinduction. The NMDA receptor antagonist CPP blocked the tetanus-induced modulation of AKAP-150 expression. Interestingly, strong generalized stimulation produced by electroconvulsive shock did not increase the expression of AKAP-150. This implies that the AKAP-150 harbours a novel property of selective responsiveness to the stimulation patterns that trigger NMDA-dependent LTP in vivo. Its selective up-regulation during LTP and its identified functions as a scaffold for protein kinase A, protein kinase C, calmodulin, calcineurin and ionotropic glutamate receptors suggest that AKAP-150 encodes is an important effector protein in the expression of late LTP.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-(2-carboxypiperazin-4-yl)propyl-1-...,
http://linkedlifedata.com/resource/pubmed/chemical/A Kinase Anchor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/Akap5 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Anticonvulsants,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Piperazines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, N-Methyl-D-Aspartate
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0953-816X
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pubmed:author |
pubmed-author:BlissT V PTV,
pubmed-author:DavisSS,
pubmed-author:DoyèreVV,
pubmed-author:ErringtonM LML,
pubmed-author:FrenchPP,
pubmed-author:GéninAA,
pubmed-author:HuntS PSP,
pubmed-author:LarocheSS,
pubmed-author:MalletJJ,
pubmed-author:MarouxSS,
pubmed-author:O'ConnorVV,
pubmed-author:Richter-LevinGG,
pubmed-author:SangerGG,
pubmed-author:SteadBB,
pubmed-author:TruchetBB,
pubmed-author:WebberMM,
pubmed-author:WillsTT
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
331-40
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:12542670-A Kinase Anchor Proteins,
pubmed-meshheading:12542670-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:12542670-Animals,
pubmed-meshheading:12542670-Anticonvulsants,
pubmed-meshheading:12542670-Carrier Proteins,
pubmed-meshheading:12542670-Electric Stimulation,
pubmed-meshheading:12542670-Electroshock,
pubmed-meshheading:12542670-Hippocampus,
pubmed-meshheading:12542670-In Situ Hybridization,
pubmed-meshheading:12542670-Long-Term Potentiation,
pubmed-meshheading:12542670-Male,
pubmed-meshheading:12542670-Neuronal Plasticity,
pubmed-meshheading:12542670-Piperazines,
pubmed-meshheading:12542670-RNA, Messenger,
pubmed-meshheading:12542670-Rats,
pubmed-meshheading:12542670-Rats, Sprague-Dawley,
pubmed-meshheading:12542670-Receptors, N-Methyl-D-Aspartate,
pubmed-meshheading:12542670-Seizures,
pubmed-meshheading:12542670-Up-Regulation
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pubmed:year |
2003
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pubmed:articleTitle |
LTP but not seizure is associated with up-regulation of AKAP-150.
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pubmed:affiliation |
Hôpital La Pitié Salpêtrière, UMR CNRS 9923, LGN, F-75013 Paris, France.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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