Linked Life Data

12539145

Source:http://linkedlifedata.com/resource/pubmed/id/12539145

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-1-22
pubmed:abstractText
We carried out uptake and depuration experiments in the laboratory to investigate the effects of temperature (15 degrees C and 25 degrees C) on the depuration kinetics and persistence of a cyanobacterial toxin, microcystin-LR (MCYST-LR), in a freshwater bivalve, Unio douglasiae. Bivalves were fed toxic Microcystis cells in the 15-day uptake experiment and nontoxic diatoms in the following 15-day depuration experiment. Each bivalve's hepatopancreas was lyophilized and extracted with a butanol:methanol:water solution for analysis of MCYST-LR by high-performance liquid chromatography. The toxin in the organ accumulated rapidly after the beginning of the uptake experiment and reached approximately steady-state conditions on day 5 at concentrations of 130 +/- 11 microg g(-1) dry weight at 15 degrees C and 250 +/- 40 microg g(-1) at 25 degrees C. In the depuration experiments MCYST-LR was eliminated asymptotically from the tissue. The values of the depuration rate constant (k(d)), calculated with a first-order one-compartment model, were found to be 0.142 +/- 0.044 day(-1) at 15 degrees C and 0.226 +/- 0.046 day(-1) at 25 degrees C. The depuration Q(10) value from 15 degrees C to 25 degrees C equaled 1.6. This study was the first to reveal the kinetics of depuration for MCYST-LR in a bivalve. The results show that MCYST-LR may be eliminated slowly in autumn and winter and persist in the tissue until spring. Thus, in terms of toxicokinetics, the risk to people of being poisoned by bivalves would increase if toxic blooms occur in autumn.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1520-4081
pubmed:author
pubmed:copyrightInfo
Copyright 2003 Wiley Periodicals, Inc.
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
61-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Depuration kinetics and persistence of the cyanobacterial toxin microcystin-LR in the freshwater bivalve Unio douglasiae.
pubmed:affiliation
Department of Environmental Sciences, Faculty of Science, Shinshu University, Matsumoto 390-8621, Japan.
pubmed:publicationType
Journal Article