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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
2003-3-24
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pubmed:abstractText |
SC1, a member of the BM-40 family of extracellular matrix proteins, was recombinantly expressed in a eukaryotic expression system. The full-length protein as well as truncated versions were purified to homogeneity under non-denaturing conditions. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry of full-length SC1 revealed a mass of 87.8 kDa of which 16.8 kDa is contributed by posttranslational modifications. In electron microscopy, after negative staining, SC1 was revealed as a globule attached to a thread-like structure. A calcium dependence of the SC1 conformation could be demonstrated by fluorescence spectroscopy. In the extracellular matrix of cultured osteosarcoma cells SC1 was found associated with collagen I-containing fibrils, and binding of SC1 to reconstituted collagen I fibrils could be demonstrated by immunogold labeling and electron microscopy. SC1 showed a broad expression in a variety of tissues.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
278
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11351-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12538579-Animals,
pubmed-meshheading:12538579-Base Sequence,
pubmed-meshheading:12538579-Calcium,
pubmed-meshheading:12538579-Calcium-Binding Proteins,
pubmed-meshheading:12538579-Circular Dichroism,
pubmed-meshheading:12538579-Collagen Type I,
pubmed-meshheading:12538579-DNA Primers,
pubmed-meshheading:12538579-Extracellular Matrix Proteins,
pubmed-meshheading:12538579-Glycoproteins,
pubmed-meshheading:12538579-Glycosylation,
pubmed-meshheading:12538579-Mice,
pubmed-meshheading:12538579-Microscopy, Fluorescence,
pubmed-meshheading:12538579-Molecular Weight,
pubmed-meshheading:12538579-Osteosarcoma,
pubmed-meshheading:12538579-Protein Binding,
pubmed-meshheading:12538579-Recombinant Proteins,
pubmed-meshheading:12538579-Spectrometry, Fluorescence,
pubmed-meshheading:12538579-Spectrometry, Mass, Matrix-Assisted Laser...,
pubmed-meshheading:12538579-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
SC1/hevin. An extracellular calcium-modulated protein that binds collagen I.
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pubmed:affiliation |
Center for Biochemistry, Medical Faculty, University of Cologne, D-50931 Cologne, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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