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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1976-6-2
|
| pubmed:abstractText |
Small samples of the 8-S species of enzymes (A1 and A2) occurring in the DNA polymerase-alpha fraction of calf thymus, have been extensively purified using non-denaturing (normal) polyacrylamide gel electrophoresis. When peak fractions of activity on normal gels were subjected to dodecylsulphate-polyacrylamide gel electrophoresis, a polypeptide at 155000 correlated with polymerase activity. Samples of the 7.3-S (C) enzyme prepared from A2 by treatment with 2.4 M urea or isolated directly without exposure to urea, also showed the presence of a 155000-Mr polypeptide. It is concluded that the 7.3-S (C) enzyme, of previously estimated molecular weight 155000-170000, is a single polypeptide and that the 8-S enzymes A1 and A2 contain an additional subunit of 50000-70000 molecular weight.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0014-2956
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
16
|
| pubmed:volume |
62
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
229-35
|
| pubmed:dateRevised |
2007-7-23
|
| pubmed:meshHeading |
pubmed-meshheading:1253788-Animals,
pubmed-meshheading:1253788-Binding Sites,
pubmed-meshheading:1253788-Cattle,
pubmed-meshheading:1253788-DNA Nucleotidyltransferases,
pubmed-meshheading:1253788-Macromolecular Substances,
pubmed-meshheading:1253788-Molecular Weight,
pubmed-meshheading:1253788-Protein Binding,
pubmed-meshheading:1253788-Thymus Gland,
pubmed-meshheading:1253788-Urea
|
| pubmed:year |
1976
|
| pubmed:articleTitle |
Evidence that DNA polymerase-alpha of calf thymus contains a subunit of molecular weight 155000.
|
| pubmed:publicationType |
Journal Article
|