Linked Life Data

12536716

Source:http://linkedlifedata.com/resource/pubmed/id/12536716

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2003-1-22
pubmed:abstractText
We investigated the cellular effect of high glucose using 3T3-L1 adipocytes on glucose transport activity, the expression of insulin signaling proteins and IRS1 tyrosine phosphorylation. Results showed that adipocytes treated with different high glucose (10, 15 and 25 mmol.L-1) for 24 hours showed to impair the basal and insulin-induced increase in glucose uptake in a dose-dependent manner and decreased significantly IRS1 tyrosine phosphorylation. High concentration of glucose produced opposite effects on IRS1 and IRS2: down-regulated IRS1 protein expression level and tightly up-regulated IRS2 contents. p85 and PKB were unaffected. Chronic exposure to high glucose can inhibit glucose uptake and induce insulin resistance. The mechanism may be involved in affecting the expression of insulin signaling peptides and tyrosine phosphorylation.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1000-5625
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
294-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2001
pubmed:articleTitle
[The molecular mechanism of high glucose-induced insulin resistance in 3T3-L1 adipocytes].
pubmed:affiliation
Department of Internal Medicine, Xiangya Hospital, Central Sooth University, Changsha 410008, China.
pubmed:publicationType
Journal Article, English Abstract