|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2003-1-20
|
|
pubmed:abstractText |
We have obtained a PrP-Cre-ER(T) transgenic mouse line (28.8) that selectively expresses in testis the tamoxifen-inducible Cre-ER(T) recombinase under the control of a mouse Prion protein (PrP) promoter-containing genomic fragment. Cre-ER(T) is expressed in spermatogonia and spermatocytes, but not in Sertoli and Leydig cells. We also established reporter PrP-L-EGFP-L transgenic mice harboring a LoxP-flanked enhanced green fluorescent protein (EGFP) Cre reporter cassette under the control of the same PrP promoter-containing genomic fragment that exhibits prominent EGFP expression in brain and testis. Using the PrP-L-EGFP-L as well as other Cre-reporter mice, we demonstrate that tamoxifen administration efficiently and selectively induces Cre-mediated recombination in the germ cell lineage. The established PrP-Cre-ER(T) line should provide a valuable tool for studying functions of germ cell-expressed genes involved in spermatogenesis.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cre recombinase,
http://linkedlifedata.com/resource/pubmed/chemical/Estrogen Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Integrases,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Plfr protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pregnancy Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Estrogen,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tamoxifen,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0006-3363
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
68
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
553-9
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:12533419-Animals,
pubmed-meshheading:12533419-Brain,
pubmed-meshheading:12533419-Cell Lineage,
pubmed-meshheading:12533419-Estrogen Antagonists,
pubmed-meshheading:12533419-Genes, Reporter,
pubmed-meshheading:12533419-Green Fluorescent Proteins,
pubmed-meshheading:12533419-Integrases,
pubmed-meshheading:12533419-Luminescent Proteins,
pubmed-meshheading:12533419-Male,
pubmed-meshheading:12533419-Mice,
pubmed-meshheading:12533419-Mice, Transgenic,
pubmed-meshheading:12533419-Mutagenesis, Site-Directed,
pubmed-meshheading:12533419-Mutation,
pubmed-meshheading:12533419-Pregnancy Proteins,
pubmed-meshheading:12533419-Protein Structure, Tertiary,
pubmed-meshheading:12533419-Receptors, Estrogen,
pubmed-meshheading:12533419-Recombinant Fusion Proteins,
pubmed-meshheading:12533419-Recombination, Genetic,
pubmed-meshheading:12533419-Spermatozoa,
pubmed-meshheading:12533419-Tamoxifen,
pubmed-meshheading:12533419-Testis,
pubmed-meshheading:12533419-Time Factors,
pubmed-meshheading:12533419-Tissue Distribution,
pubmed-meshheading:12533419-Viral Proteins
|
|
pubmed:year |
2003
|
|
pubmed:articleTitle |
Temporally controlled site-specific mutagenesis in the germ cell lineage of the mouse testis.
|
|
pubmed:affiliation |
Institut de Génétique et de Biologie Moléculaire et Cellulaire, Université Louis Pasteur, Collège de France, B P 163, 67404 Illkirch-Cedex, Communauté Urbaine de Strasbourg, France.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
