Linked Life Data

12531475

Source:http://linkedlifedata.com/resource/pubmed/id/12531475

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2003-1-17
pubmed:abstractText
In some cases core histone genes in the mouse depend on intragenic sequence elements for high level expression [Gene 176 (1996) 1]. Here we report that the highly expressed gene for rat linker histone H1d also contains an intragenic activating region (IAR). Using transient transfection assays in mouse fibroblast NIH3T3 cells, we showed that rat H1d contains a downstream region (+21 to +116) that imparts a two- to threefold up-regulation of fused reporters. This region also activated expression when moved to the promoter region, though the effect was dependent on its distance from other promoter elements. The IAR contains sequence homologies to the core alpha and Omega elements identified as functional protein binding sites within the mouse H3.2 coding region activating sequence (CRAS). A pair of Omega elements (+32 and +66) accounts for the activating effect of the H1d intragenic region as shown by targeted mutations as well as stepwise deletions. The H1d and H3.2 Omega sequences bound similar and perhaps identical proteins by gel shift analysis. The H1d alpha-like sequence at +56 overlaps the translational start codon and was therefore not mutated. Like the mouse H3.2 alpha element, it bound transcription factor YY1 in gel shift assays. H1t, the gene for the testis-specific linker histone, did not demonstrate an IAR. While H1t has a similar alpha sequence and did bind YY1, it lacks the Omega homologies of H1d. Sequence comparison shows that the YY1/alpha site as well as the adjacent Omega site are likely present in genes for other standard H1 variants, but that the +32 Omega site in the 5' untranslated region (UTR) of H1d is unique. We conclude that the +32 and +66 Omega sequences of the rat H1d gene contribute significantly to its high-level expression.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
1625
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
165-72
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:12531475-3T3 Cells, pubmed-meshheading:12531475-Animals, pubmed-meshheading:12531475-Base Sequence, pubmed-meshheading:12531475-Binding Sites, pubmed-meshheading:12531475-DNA-Binding Proteins, pubmed-meshheading:12531475-Electrophoretic Mobility Shift Assay, pubmed-meshheading:12531475-Erythroid-Specific DNA-Binding Factors, pubmed-meshheading:12531475-Genes, Reporter, pubmed-meshheading:12531475-Genetic Variation, pubmed-meshheading:12531475-Histones, pubmed-meshheading:12531475-Male, pubmed-meshheading:12531475-Mice, pubmed-meshheading:12531475-Molecular Sequence Data, pubmed-meshheading:12531475-Nuclear Proteins, pubmed-meshheading:12531475-Promoter Regions, Genetic, pubmed-meshheading:12531475-Rats, pubmed-meshheading:12531475-Testis, pubmed-meshheading:12531475-Transcription Factors, pubmed-meshheading:12531475-Transcription Initiation Site, pubmed-meshheading:12531475-Transfection, pubmed-meshheading:12531475-YY1 Transcription Factor, pubmed-meshheading:12531475-beta-Galactosidase
pubmed:year
2003
pubmed:articleTitle
The rat histone H1d gene has intragenic activating sequences that are absent from the testis-specific variant H1t.
pubmed:affiliation
Department of Chemistry and Biochemistry, University of South Carolina, GSRC, 631 Sumter St., Columbia, SC 29208, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.