Source:http://linkedlifedata.com/resource/pubmed/id/12531199
Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
2003-1-17
|
pubmed:abstractText |
Xenobiotic carboxylic acids, that via their metabolites covalently modify proteins, have been associated with serious side effects in man. Such reactive metabolites may be acyl glucuronides or alternatively, the corresponding acyl-CoA thioesters. In this study, the reaction of a model xenobiotic acyl-CoA, the naproxen-CoA, with human serum albumin (HSA), was characterized by high-performance liquid chromatography employing fluorescence and mass spectrometric detection. One mM naproxen-CoA was incubated for 6h with HSA (0.45 mM) at 37 degrees C in a 0.1M phosphate buffer (pH 7.4). The tryptic digest of the reduced and alkylated protein was analyzed in order to identify the amino acids in the sequence that were covalently modified with naproxen. Fluorescent peptides, that represented naproxen-modified peptides, were characterized using HPLC-MS-MS and HPLC-MS in zoom scan mode, which provided information on the structure and the charge of the modified peptides. The naproxen-CoA reacted predominantly with lysine 199, lysine 541, and lysine 351, which was in agreement with the binding pattern that has previously been reported for the reactive acyl glucuronides and their reaction with HSA.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jan
|
pubmed:issn |
0003-2697
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
312
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
148-56
|
pubmed:dateRevised |
2006-11-15
|
pubmed:meshHeading |
pubmed-meshheading:12531199-Acyl Coenzyme A,
pubmed-meshheading:12531199-Amino Acid Sequence,
pubmed-meshheading:12531199-Amino Acids,
pubmed-meshheading:12531199-Catalytic Domain,
pubmed-meshheading:12531199-Chromatography, High Pressure Liquid,
pubmed-meshheading:12531199-Fluorescence,
pubmed-meshheading:12531199-Humans,
pubmed-meshheading:12531199-Mass Spectrometry,
pubmed-meshheading:12531199-Molecular Sequence Data,
pubmed-meshheading:12531199-Molecular Weight,
pubmed-meshheading:12531199-Naproxen,
pubmed-meshheading:12531199-Serum Albumin
|
pubmed:year |
2003
|
pubmed:articleTitle |
Identification of the amino acids of human serum albumin involved in the reaction with the naproxen acyl coenzyme A thioester using liquid chromatography combined with fluorescence and mass spectrometric detection.
|
pubmed:affiliation |
Department of Analytical and Pharmaceutical Chemistry, The Royal Danish School of Pharmacy, Universitetsparken 2, DK-2100 Copenhagen, Denmark. jol@dfh.dk
|
pubmed:publicationType |
Journal Article
|