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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2003-1-16
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pubmed:abstractText |
Posttranscriptional controls play a major role in eucaryotic gene expression. These controls are mediated by sequence-specific interactions of cis-acting determinants in target mRNAs with one or more protein factors. The positioning of a subset of these mRNA-protein (RNP) complexes within the 3' untranslated region (3' UTR) may allow them to remain associated with the mRNA during active translation. Robust expression of human alpha-globin mRNA during erythroid differentiation has been linked to formation of a binary complex between a KH-domain protein, alphaCP, and a 3' UTR C-rich motif. Detection of this "alpha-complex" has been limited to in vitro studies, and the functional state of the alpha-globin mRNA targeted by alphaCP has not been defined. In the present study we demonstrate that a significant fraction of alphaCP is associated with polysomal mRNA. Targeted analysis of the polysomal RNP complexes revealed that alphaCP is specifically bound to actively translating alpha-globin mRNA. The bound alphaCP is restricted to the poly(C)-rich 3' UTR motif and is dislodged when ribosomes are allowed to enter this region. These data validate the general importance of the 3' UTR as a sheltered site for RNP complexes and support a specific model in which the stabilizing function of alphaCP is mediated on actively translating target mRNAs.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12529395-10373504,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12529395-9827798
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0270-7306
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
899-907
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:12529395-3' Untranslated Regions,
pubmed-meshheading:12529395-Animals,
pubmed-meshheading:12529395-Binding Sites,
pubmed-meshheading:12529395-Carrier Proteins,
pubmed-meshheading:12529395-Cell Line,
pubmed-meshheading:12529395-Globins,
pubmed-meshheading:12529395-Humans,
pubmed-meshheading:12529395-Mice,
pubmed-meshheading:12529395-Models, Biological,
pubmed-meshheading:12529395-Polyribosomes,
pubmed-meshheading:12529395-Protein Biosynthesis,
pubmed-meshheading:12529395-RNA, Messenger,
pubmed-meshheading:12529395-RNA Processing, Post-Transcriptional,
pubmed-meshheading:12529395-RNA Stability,
pubmed-meshheading:12529395-Ribonucleoproteins
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pubmed:year |
2003
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pubmed:articleTitle |
In vivo association of the stability control protein alphaCP with actively translating mRNAs.
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pubmed:affiliation |
Department of Genetics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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