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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2003-1-13
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pubmed:abstractText |
Mutations in the gene for Su(var)3-9 are dominant suppressors of position-effect variegation (PEV). We show that SU(VAR)3-9 is a chromatin-associated protein and identify the large multicopy histone gene cluster (HIS-C) as one of its target loci. The organization of nucleosomes over the entire HIS-C region is altered in Su(var)3-9 mutants and there is a concomitant increase in expression of the histone genes. SU(VAR)3-9 is a histone H3 methyltransferase and, using chromatin immunoprecipitation, we show that SU(VAR)3-9 is present at the HIS-C locus and that the histone H3 at the HIS-C locus is methylated. We propose that SU(VAR)3-9 is involved in packaging HIS-C into a distinct chromatin domain that has some of the characteristics of beta-heterochromatin. We suggest that methylation of histone H3 is important for the chromatin structure at HIS-C. The chromosomal deficiency for the HIS-C is also a suppressor of PEV. In contrast to what might be expected, we show that hemizygosity for the HIS-C locus leads to a substantial increase in the histone transcripts.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-10209020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-10393187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-10586882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-10949293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-11124534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-11283354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-11498594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-11566886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-116133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-11867540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-12086617,
http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-1482113,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/12524347-9832530
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Histone-Lysine N-Methyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Methyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Methyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SU(VAR)3-9,
http://linkedlifedata.com/resource/pubmed/chemical/Suppressor of variegation 3-9...,
http://linkedlifedata.com/resource/pubmed/chemical/histone methyltransferase
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0016-6731
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1763-74
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pubmed:dateRevised |
2010-9-14
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pubmed:meshHeading |
pubmed-meshheading:12524347-Animals,
pubmed-meshheading:12524347-Base Sequence,
pubmed-meshheading:12524347-Chromatin,
pubmed-meshheading:12524347-DNA,
pubmed-meshheading:12524347-Drosophila,
pubmed-meshheading:12524347-Drosophila Proteins,
pubmed-meshheading:12524347-Female,
pubmed-meshheading:12524347-Gene Expression Regulation, Developmental,
pubmed-meshheading:12524347-Genes, Insect,
pubmed-meshheading:12524347-Histone-Lysine N-Methyltransferase,
pubmed-meshheading:12524347-Histones,
pubmed-meshheading:12524347-Male,
pubmed-meshheading:12524347-Methyltransferases,
pubmed-meshheading:12524347-Multigene Family,
pubmed-meshheading:12524347-Mutation,
pubmed-meshheading:12524347-Protein Methyltransferases,
pubmed-meshheading:12524347-RNA, Messenger,
pubmed-meshheading:12524347-Repressor Proteins,
pubmed-meshheading:12524347-Suppression, Genetic
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pubmed:year |
2002
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pubmed:articleTitle |
A role for the Drosophila SU(VAR)3-9 protein in chromatin organization at the histone gene cluster and in suppression of position-effect variegation.
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pubmed:affiliation |
Department of Zoology, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada. ner@zoology.ubc.ca
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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