Linked Life Data

12523646

Source:http://linkedlifedata.com/resource/pubmed/id/12523646

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2003-1-13
pubmed:databankReference
pubmed:abstractText
A cDNA encoding for a copper containing amine oxidase has been isolated and sequenced from young leaves of Euphorbia characias, a perennial mediterranean shrub. A single long open reading frame of 2068 pb encodes a protein composed of 653 amino acids with a molecular mass of about 74 kDa. A putative 24-aminoacid signal peptide precedes the sequence of the mature protein, with characteristics of a secretion signal peptide. Alignments of Euphorbia amine oxidase cDNA nucleotide sequence with that of amine oxidase from the seedlings of the pulses lentil, pea, and chickpea reveal several conserved regions, especially in the C-terminus, with a homology 90%-97%. The near 5' region shows several insertions, deletions, and different nucleotide sequence with ca. 60% homology. The enzyme contains 1%-2% carbohydrate deduced by deglycosylation experiments. Five cysteine residues are present in the deduced aminoacid sequence with a single disulfide bridge as judged by titration with cysteine reagents.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0277-8033
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
435-41
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2002
pubmed:articleTitle
Structure and nucleotide sequence of Euphorbia characias copper/TPQ-containing amine oxidase gene.
pubmed:affiliation
Department of Sciences Applied to Biosystems, University of Cagliari, Cagliari, Italy. padiglia@unica.it
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't