Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-1-10
pubmed:abstractText
1 The present study was performed to evaluate the presence and the physiological consequences of butyrylcholinesterase (BChE) inhibition on isolated phrenic-hemidiaphragm preparations from normal mice expressing acetylcholinesterase (AChE) and BChE, and from AChE-knockout mice (AChE(-/-)) expressing only BChE. 2 Histochemical and enzymatic assays revealed abundance of AChE and BChE in normal mature neuromuscular junctions (NMJs). 3 In normal NMJs, in which release was reduced by low Ca(2+)/high Mg(2+) medium BChE inhibition with tetraisopropylpyrophosphoramide (iso-OMPA) or bambuterol decreased ( approximately 50%) evoked quantal release, while inhibition of AChE with fasciculin-1, galanthamine (10, 20 micro M) or neostigmine (0.1-1 micro M) increased (50-80%) evoked quantal release. Inhibition of both AChE and BChE with galanthamine (80 micro M), neostigmine (3-10 micro M), O-ethylS-2-(diisopropylamino)ethyl-methylphosphono-thioate (MTP) or phospholine decreased evoked transmitter release (20-50%). 4 In AChE(-/-) NMJs, iso-OMPA pre-treatment decreased evoked release. 5 Muscarinic toxin-3 decreased evoked release in both AChE(-/-) and normal NMJs treated with low concentrations of neostigmine, galanthamine or fasciculin-1, but had no effect in normal NMJs pretreated with iso-OMPA, bambuterol, MTP and phospholine. 6 In normal and AChE(-/-) NMJs pretreatment with iso-OMPA failed to affect the time course of miniature endplate potentials and full-sized endplate potentials. 7 Overall, our results suggest that inhibition or absence of AChE increases evoked quantal release by involving muscarinic receptors (mAChRs), while BChE inhibition decreases release through direct or indirect mechanisms not involving mAChRs. BChE apparently is not implicated in limiting the duration of acetylcholine action on postsynaptic receptors, but is involved in a presynaptic modulatory step of the release process.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-10087275, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-10665486, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-10869390, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-10936216, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-11753420, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-11876771, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-11961141, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-12093157, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-12128253, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-13726518, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-14784953, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-1662770, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-1977361, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-1999397, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-2125333, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-229216, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-2365076, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-2390674, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-2669736, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-2709330, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-3817286, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-4178052, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-4361216, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-552605, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-6127680, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-6267255, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-648523, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-6966689, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-7914535, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-7925952, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-7938228, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-8321908, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-8485630, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-8499198, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-8554068, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-9222557, http://linkedlifedata.com/resource/pubmed/commentcorrection/12522088-9647869
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
177-87
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Butyrylcholinesterase and acetylcholinesterase activity and quantal transmitter release at normal and acetylcholinesterase knockout mouse neuromuscular junctions.
pubmed:affiliation
Institut Fédératif de Neurobiologie Alfred Fessard, Laboratoire de Neurobiologie Cellulaire et Moléculaire, U.P.R. 9040 C.N.R.S., 1 avenue de la Terrasse, 91198 Gif-sur-Yvette Cedex, France.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't