Source:http://linkedlifedata.com/resource/pubmed/id/12518855
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
2003-1-9
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pubmed:databankReference | |
pubmed:abstractText |
In this work, we report the partial sequence of a 51 kDa protein of Entamoeba histolytica that is highly immunogenic in humans. Partial sequencing of the N-terminal end showed that 18 of the first 20 amino acid residues of the protein were identified uniquely, indicating that the final product was a homogeneous protein preparation. The N-terminal sequence that was found was: KVYFEETFENGWKXIWSKW. Comparing the 19-amino acid sequence of the protein in automated databases shows significant similarity with amino acid sequences of the calreticulin-like protein of spinach leaves (77%) and of the calreticulin precursor of Dictyostelium discoideum (60%).
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0002-9637
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
67
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
636-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12518855-Amino Acid Sequence,
pubmed-meshheading:12518855-Animals,
pubmed-meshheading:12518855-Antibodies, Protozoan,
pubmed-meshheading:12518855-Blotting, Western,
pubmed-meshheading:12518855-Calreticulin,
pubmed-meshheading:12518855-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12518855-Entamoeba histolytica,
pubmed-meshheading:12518855-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:12518855-Humans,
pubmed-meshheading:12518855-Molecular Sequence Data,
pubmed-meshheading:12518855-Protozoan Proteins,
pubmed-meshheading:12518855-Sequence Analysis, Protein
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pubmed:year |
2002
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pubmed:articleTitle |
Calreticulin-like molecule in trophozoites of Entamoeba histolytica HM1:IMSS (Swissprot: accession P83003).
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pubmed:affiliation |
Depto. de Medicina Experimental, Facultad de Medicine, UNAM, México, DF, México.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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