| pubmed-article:12518221 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:12518221 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:12518221 | lifeskim:mentions | umls-concept:C1412112 | lld:lifeskim |
| pubmed-article:12518221 | lifeskim:mentions | umls-concept:C1420305 | lld:lifeskim |
| pubmed-article:12518221 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:12518221 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:12518221 | lifeskim:mentions | umls-concept:C1705920 | lld:lifeskim |
| pubmed-article:12518221 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:12518221 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:12518221 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:12518221 | pubmed:dateCreated | 2003-1-8 | lld:pubmed |
| pubmed-article:12518221 | pubmed:abstractText | The homeodomain protein, Cdx-2, as transcription factor has been implicated in the transcriptional regulation of genes expressed in small intestine and the process of tumorgenesis. In current work, a conserved mouse Cdx-2 domain (mCdx-2D) coded by its cDNA fragment, which was amplified and cloned into the expression vector pGEX-4T1, was expressed as a fusion protein with GST (GST-mCd x-2D) and purified by one step of affinity chromatography. A polyclonal antibody against Cdx-2 was raised by using the recombinant fusion protein GST-mCdx-2D as antigen and was fractionated from the rabbit anti-serum. Western blot and EMSA (electrophoretic mobility shift assay) demonstrate that the natural and denatured Cdx-2s from different species (mouse and human) can be detected by the prepared anti-Cdx-2 antibody. Most notably, we found that the Cdx-2 in human intestine cell line Caco-2 is expressed in a differentiation-dependent manner and can efficiently bind to the mouse and human acat2 (acyl-coenzyme A: cholesterol acyltransferase 2) promoter regions, suggesting that the transcriptional factor Cdx-2 may play a role in regulating the acat2 expression in the intestinal cells. | lld:pubmed |
| pubmed-article:12518221 | pubmed:language | eng | lld:pubmed |
| pubmed-article:12518221 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12518221 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:12518221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12518221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12518221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12518221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12518221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12518221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12518221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12518221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12518221 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:12518221 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:12518221 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:12518221 | pubmed:issn | 0582-9879 | lld:pubmed |
| pubmed-article:12518221 | pubmed:author | pubmed-author:YuY FYF | lld:pubmed |
| pubmed-article:12518221 | pubmed:author | pubmed-author:LiBo-LiangBL | lld:pubmed |
| pubmed-article:12518221 | pubmed:author | pubmed-author:LinZhi-XinZX | lld:pubmed |
| pubmed-article:12518221 | pubmed:author | pubmed-author:YangXin-YingX... | lld:pubmed |
| pubmed-article:12518221 | pubmed:author | pubmed-author:SongBao-Liang... | lld:pubmed |
| pubmed-article:12518221 | pubmed:author | pubmed-author:WangCan-HuaCH | lld:pubmed |
| pubmed-article:12518221 | pubmed:author | pubmed-author:YangJin-BoJB | lld:pubmed |
| pubmed-article:12518221 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:12518221 | pubmed:volume | 35 | lld:pubmed |
| pubmed-article:12518221 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:12518221 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:12518221 | pubmed:pagination | 6-12 | lld:pubmed |
| pubmed-article:12518221 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:12518221 | pubmed:meshHeading | pubmed-meshheading:12518221... | lld:pubmed |
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| pubmed-article:12518221 | pubmed:meshHeading | pubmed-meshheading:12518221... | lld:pubmed |
| pubmed-article:12518221 | pubmed:year | 2003 | lld:pubmed |
| pubmed-article:12518221 | pubmed:articleTitle | Preparation of an anti-Cdx-2 antibody for analysis of different species Cdx-2 binding to acat2 promoter. | lld:pubmed |
| pubmed-article:12518221 | pubmed:affiliation | State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China. | lld:pubmed |
| pubmed-article:12518221 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:12518221 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:39 | entrezgene:pubmed | pubmed-article:12518221 | lld:entrezgene |
| entrez-gene:1045 | entrezgene:pubmed | pubmed-article:12518221 | lld:entrezgene |
| http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:12518221 | lld:entrezgene |
