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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2003-1-8
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pubmed:abstractText |
The homeodomain protein, Cdx-2, as transcription factor has been implicated in the transcriptional regulation of genes expressed in small intestine and the process of tumorgenesis. In current work, a conserved mouse Cdx-2 domain (mCdx-2D) coded by its cDNA fragment, which was amplified and cloned into the expression vector pGEX-4T1, was expressed as a fusion protein with GST (GST-mCd x-2D) and purified by one step of affinity chromatography. A polyclonal antibody against Cdx-2 was raised by using the recombinant fusion protein GST-mCdx-2D as antigen and was fractionated from the rabbit anti-serum. Western blot and EMSA (electrophoretic mobility shift assay) demonstrate that the natural and denatured Cdx-2s from different species (mouse and human) can be detected by the prepared anti-Cdx-2 antibody. Most notably, we found that the Cdx-2 in human intestine cell line Caco-2 is expressed in a differentiation-dependent manner and can efficiently bind to the mouse and human acat2 (acyl-coenzyme A: cholesterol acyltransferase 2) promoter regions, suggesting that the transcriptional factor Cdx-2 may play a role in regulating the acat2 expression in the intestinal cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0582-9879
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6-12
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12518221-Amino Acid Sequence,
pubmed-meshheading:12518221-Animals,
pubmed-meshheading:12518221-Antibodies, Monoclonal,
pubmed-meshheading:12518221-Antibody Specificity,
pubmed-meshheading:12518221-Binding Sites,
pubmed-meshheading:12518221-CHO Cells,
pubmed-meshheading:12518221-Caco-2 Cells,
pubmed-meshheading:12518221-Cricetinae,
pubmed-meshheading:12518221-DNA Probes,
pubmed-meshheading:12518221-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:12518221-Electrophoretic Mobility Shift Assay,
pubmed-meshheading:12518221-Glutathione Transferase,
pubmed-meshheading:12518221-HeLa Cells,
pubmed-meshheading:12518221-Homeodomain Proteins,
pubmed-meshheading:12518221-Humans,
pubmed-meshheading:12518221-Mice,
pubmed-meshheading:12518221-Molecular Sequence Data,
pubmed-meshheading:12518221-Promoter Regions, Genetic,
pubmed-meshheading:12518221-Protein Binding,
pubmed-meshheading:12518221-Recombinant Fusion Proteins,
pubmed-meshheading:12518221-Sequence Homology, Amino Acid,
pubmed-meshheading:12518221-Sterol O-Acyltransferase,
pubmed-meshheading:12518221-Trans-Activators,
pubmed-meshheading:12518221-Tumor Cells, Cultured
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pubmed:year |
2003
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pubmed:articleTitle |
Preparation of an anti-Cdx-2 antibody for analysis of different species Cdx-2 binding to acat2 promoter.
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pubmed:affiliation |
State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200031, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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