Source:http://linkedlifedata.com/resource/pubmed/id/12517929
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
2003-1-8
|
| pubmed:abstractText |
Humoral immune responses elicited after secondary exposure to immunizing Ag are characterized by robust and elevated reactivity of memory B cells that exceed those of naive B cells during the primary response. The mechanism underlying this difference in responsiveness of naive vs memory B cells remains unclear. We have quantitated the response of naive and memory human B cells after in vitro stimulation with T cell-derived stimuli. In response to stimulation with CD40 ligand alone or with IL-10, both IgM-expressing and Ig isotype-switched memory B cells entered their first division 20-30 h earlier than did naive B cells. In contrast, the time spent traversing subsequent divisions was similar. Consistent with previous studies, only memory cells differentiated to CD38(+) blasts in a manner that increased with consecutive division number. These differentiated CD38(+) B cells divided faster than did CD38(-) memory B cell blasts. Proliferation of CD40 ligand-stimulated naive B cells as well as both CD38(+) and CD38(-) cells present in cultures of memory B cells was increased by IL-10. In contrast, IL-2 enhanced proliferation of CD38(-) and CD38(+) memory B cell blasts, but not naive cells. Thus, memory B cells possess an intrinsic advantage over naive B cells in both the time to initiate a response and in the division-based rate of effector cell development. These differences help explain the accelerated Ab response exhibited by memory B cells after secondary challenge by an invading pathogen, a hallmark of immunological memory.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosyl Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD27,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/Bromodeoxyuridine,
http://linkedlifedata.com/resource/pubmed/chemical/CD38 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-2
|
| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
170
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
686-94
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:12517929-ADP-ribosyl Cyclase,
pubmed-meshheading:12517929-Antigens, CD,
pubmed-meshheading:12517929-Antigens, CD27,
pubmed-meshheading:12517929-Antigens, CD38,
pubmed-meshheading:12517929-B-Lymphocyte Subsets,
pubmed-meshheading:12517929-Bromodeoxyuridine,
pubmed-meshheading:12517929-Cell Differentiation,
pubmed-meshheading:12517929-Cell Division,
pubmed-meshheading:12517929-Cells, Cultured,
pubmed-meshheading:12517929-Humans,
pubmed-meshheading:12517929-Immunization, Secondary,
pubmed-meshheading:12517929-Immunologic Memory,
pubmed-meshheading:12517929-Immunophenotyping,
pubmed-meshheading:12517929-Interphase,
pubmed-meshheading:12517929-Kinetics,
pubmed-meshheading:12517929-Lymphocyte Activation,
pubmed-meshheading:12517929-Membrane Glycoproteins,
pubmed-meshheading:12517929-Receptors, Interleukin-2,
pubmed-meshheading:12517929-Time Factors
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| pubmed:year |
2003
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| pubmed:articleTitle |
Intrinsic differences in the proliferation of naive and memory human B cells as a mechanism for enhanced secondary immune responses.
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| pubmed:affiliation |
Immune Regulation Group, Centenary Institute of Cancer Medicine and Cell Biology, Sydney, Australia. s.tangye@centenary.usyd.edu.au
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|