Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2003-1-8
pubmed:abstractText
We have developed nucleic acid sequence-based amplification (NASBA), standard reverse transcription PCR (RT-PCR), and TaqMan nucleic acid amplification assays for the rapid detection of North American eastern equine encephalitis (EEE) and western equine encephalitis (WEE) viral RNAs from samples collected in the field and clinical samples. The sensitivities of these assays have been compared to that of virus isolation. While all three types of nucleic acid amplification assays provide rapid detection of viral RNAs comparable to the isolation of viruses in Vero cells, the TaqMan assays for North American EEE and WEE viral RNAs are the most sensitive. We have shown these assays to be specific for North American EEE and WEE viral RNAs by testing geographically and temporally distinct strains of EEE and WEE viruses along with a battery of related and unrelated arthropodborne viruses. In addition, all three types of nucleic acid amplification assays have been used to detect North American EEE and WEE viral RNAs from mosquito and vertebrate tissue samples. The sensitivity, specificity, and rapidity of nucleic acid amplification demonstrate the usefulness of NASBA, standard RT-PCR, and TaqMan assays, in both research and diagnostic settings, to detect North American EEE and WEE viral RNAs.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-10548292, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-11060069, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-11283039, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-11337046, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-11724870, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-11804455, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-11931239, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-14151098, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-1726172, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-2158755, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-2574935, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-6265396, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-8254725, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-8867585, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-8908134, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-93518, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-9547273, http://linkedlifedata.com/resource/pubmed/commentcorrection/12517876-9889212
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
379-85
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Detection of North American eastern and western equine encephalitis viruses by nucleic acid amplification assays.
pubmed:affiliation
Division of Vector-Borne Infectious Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Fort Collins, Colorado 80521, USA. ahk7@cdc.gov
pubmed:publicationType
Journal Article, Comparative Study