12515806

Source:http://linkedlifedata.com/resource/pubmed/id/12515806

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0025914, umls-concept:C0026809, umls-concept:C0033684, umls-concept:C0085304, umls-concept:C0439851, umls-concept:C0767136, umls-concept:C1167622, umls-concept:C1179464, umls-concept:C1419142, umls-concept:C1449624, umls-concept:C1552596, umls-concept:C1947931
pubmed:issue	8
pubmed:dateCreated	2003-2-17
pubmed:abstractText	PAR-3 is a cell polarity protein that localizes at tight junctions (TJs) by direct binding to an immunoglobulin (Ig)-like cell-cell adhesion molecule JAM-1 in mammalian epithelial cells. Another Ig-like cell-cell adhesion molecule nectin plays a role in the localization of JAM-1 at TJs in epithelial cells. Nectin furthermore plays a role in the organization of adherens junctions (AJs) and TJs. Nectin comprises a family of four members, nectin-1, -2, -3, and -4. Nectins are associated with the actin cytoskeleton through afadin, of which the PDZ domain binds to nectins through their C-terminal four amino acids. We show here that PAR-3 binds to nectin-1 and -3 in neuroepithelial cells of the embryonic telencephalon, which are equipped with AJs, but not with typical TJs. Nectin-1, -2, -3, and afadin, but not JAM-1, were concentrated at AJs in neuroepithelial cells of the embryonic telencephalon at E13.5 and PAR-3 co-localized with nectins. PAR-3 was co-immunoprecipitated with nectin-1 and -3, but not with nectin-2 or JAM-1, from the mouse whole brain at E13.5. Recombinant PAR-3 stoichiometrically bound to recombinant nectin-1 and -3. The first one of the three PDZ domains of PAR-3 bound to the C-terminal four amino acids of nectin-1 and -3. The affinities of PAR-3 and afadin for nectin-1 and -3 were similar. Cadherin-deficient L cells expressing nectin-1 and -3 formed nectin-1- and -3-based cell-cell junctions, respectively, where PAR-3 as well as afadin was recruited. These results indicate that nectin-1 and -3 are involved in the localization of PAR-3 at AJs in the neuroepithelial cells of the embryonic telencephalon.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/2985121R
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules, http://linkedlifedata.com/resource/pubmed/chemical/Intermediate Filament Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Kinesin, http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Mllt4 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Myosins, http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Pard3 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/afadin, http://linkedlifedata.com/resource/pubmed/chemical/nestin
pubmed:status	MEDLINE
pubmed:month	Feb
pubmed:issn	0021-9258
pubmed:author	pubmed-author:FujitoTsutomuT, pubmed-author:IkedaWataruW, pubmed-author:MondenMoritoM, pubmed-author:MorimotoKojiK, pubmed-author:TakaiYoshimiY, pubmed-author:TakekuniKyojiK, pubmed-author:TakeuchiMasakazuM
pubmed:issnType	Print
pubmed:day	21
pubmed:volume	278
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	5497-500
pubmed:dateRevised	2009-11-19
pubmed:meshHeading	pubmed-meshheading:12515806-Animals, pubmed-meshheading:12515806-Brain, pubmed-meshheading:12515806-Carrier Proteins, pubmed-meshheading:12515806-Cell Adhesion Molecules, pubmed-meshheading:12515806-Cell Polarity, pubmed-meshheading:12515806-Epithelial Cells, pubmed-meshheading:12515806-Intermediate Filament Proteins, pubmed-meshheading:12515806-Kinesin, pubmed-meshheading:12515806-L Cells (Cell Line), pubmed-meshheading:12515806-Mice, pubmed-meshheading:12515806-Microfilament Proteins, pubmed-meshheading:12515806-Microscopy, Immunoelectron, pubmed-meshheading:12515806-Myosins, pubmed-meshheading:12515806-Nerve Tissue Proteins, pubmed-meshheading:12515806-Neurons, pubmed-meshheading:12515806-Protein Binding, pubmed-meshheading:12515806-Recombinant Proteins, pubmed-meshheading:12515806-Sequence Deletion, pubmed-meshheading:12515806-Tight Junctions
pubmed:year	2003
pubmed:articleTitle	Direct binding of cell polarity protein PAR-3 to cell-cell adhesion molecule nectin at neuroepithelial cells of developing mouse.
pubmed:affiliation	Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita 565-0871, Japan.
pubmed:publicationType	Journal Article, Comparative Study, Research Support, Non-U.S. Gov't