Linked Life Data

12512599

Source:http://linkedlifedata.com/resource/pubmed/id/12512599

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2003-1-6
pubmed:abstractText
Lactating Holstein cows, averaging 80 d in milk, were used to examine effects of exogenous bovine somatotropin (bST) on oviductal and uterine genes encoding components of the insulin-like growth factor (IGF) system. About 12 h before expected ovulation in an Ovsynch protocol, cows were assigned randomly to receive bST (500 mg; n = 11) or serve as untreated controls (n = 10). Cows that ovulated (n = 9 bST, 8 control) were divided within treatment to be sacrificed on d 3 or 7 postovulation. Samples of oviductal and intercaruncular endometrial tissue from oviducts and uterine horns ipsilateral to the corpus luteum (CL) were collected and immediately frozen at -80 degrees C for subsequent mRNA analyses. Northern blots revealed mRNAs for IGF-II, IGF-binding protein-2 (IGFBP-2), and IGFBP-3 in all oviductal and endometrial tissues. Significant amounts of IGF-I and growth hormone receptor-1A (GHR-1A) mRNAs were detected in uteri but not in oviducts. The bST treatment had no effect on amount of IGF-I mRNA transcript in uterine endometrium. The mRNA encoding IGF-II was induced by bST in oviducts collected on both d 3 and 7 but was down-regulated in endometrium on d 7. Transcript of IGFBP-2 mRNA was greater in endometrial than oviductal tissues and did not differ between treatments. Both oviductal and endometrial IGFBP-3 mRNA concentrations increased between d 3 and 7 postovulation, with a tendency for greater endometrial IGFBP-3 mRNA in bST-treated cows on d 7. On d 7, concentrations of endometrial GHR-1A mRNA were 30% lower in bST-treated cows. Results indicate complex and tissue-specific regulation of the uterine IGF system components by exogenous bST. Some of those biological responses to bST may be important in early development of bovine embryos.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-0302
pubmed:author
pubmed:issnType
Print
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3260-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:12512599-Animals, pubmed-meshheading:12512599-Blotting, Northern, pubmed-meshheading:12512599-Cattle, pubmed-meshheading:12512599-Endometrium, pubmed-meshheading:12512599-Estrous Cycle, pubmed-meshheading:12512599-Fallopian Tubes, pubmed-meshheading:12512599-Female, pubmed-meshheading:12512599-Gene Expression, pubmed-meshheading:12512599-Growth Hormone, pubmed-meshheading:12512599-Insulin-Like Growth Factor Binding Protein 2, pubmed-meshheading:12512599-Insulin-Like Growth Factor Binding Protein 3, pubmed-meshheading:12512599-Insulin-Like Growth Factor I, pubmed-meshheading:12512599-Insulin-Like Growth Factor II, pubmed-meshheading:12512599-Lactation, pubmed-meshheading:12512599-RNA, Messenger, pubmed-meshheading:12512599-Receptors, Somatotropin, pubmed-meshheading:12512599-Somatomedins, pubmed-meshheading:12512599-Uterus
pubmed:year
2002
pubmed:articleTitle
Effects of BST on oviductal and uterine genes encoding components of the IGF system in lactating dairy cows.
pubmed:affiliation
Department of Animal Sciences University of Florida, Gainesville 32611, USA.
pubmed:publicationType
Journal Article, Clinical Trial, Research Support, U.S. Gov't, Non-P.H.S., Randomized Controlled Trial