Linked Life Data

12511180

Source:http://linkedlifedata.com/resource/pubmed/id/12511180

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2003-1-3
pubmed:abstractText
The present study was performed to evaluate the role of neuronal nitric oxide synthase (nNOS)-derived nitric oxide (NO) during the developmental phase of hypertension in transgenic rats harboring the mouse Ren-2 renin gene (TGR). The first aim of the present study was to examine nNOS mRNA expression in the renal cortex and to assess the renal functional responses to intrarenal nNOS inhibition by S-methyl-L-thiocitrulline (L-SMTC) in heterozygous TGR and in age-matched transgene-negative Hannover Sprague-Dawley rats (HanSD). The second aim was to evaluate the role of the renal sympathetic nerves in mediating the renal functional responses to intrarenal nNOS inhibition. Thus, we also evaluated the effects of intrarenal L-SMTC administration in acutely denervated TGR and HanSD. Expression of nNOS mRNA in the renal cortex was significantly increased in TGR compared with HanSD. Intrarenal administration of L-SMTC decreased the glomerular filtration rate (GFR), renal plasma flow (RPF) and sodium excretion and increased renal vascular resistance (RVR) in HanSD. In contrast, intrarenal inhibition of nNOS by L-SMTC did not alter GFR, RPF or RVR and elicited a marked increase in sodium excretion in TGR. This effect of intrarenal L-SMTC was not observed in acutely denervated TGR. These results suggest that during the developmental phase of hypertension TGR exhibit an impaired renal vascular responsiveness to nNOS derived NO or an impaired ability to release NO by nNOS despite enhanced expression of nNOS mRNA in the renal cortex. In addition, the data indicate that nNOS-derived NO increases tubular sodium reabsorption in TGR and that the renal nerves play an important modulatory role in this process.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0862-8408
pubmed:author
pubmed:issnType
Print
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
571-80
pubmed:dateRevised
2008-4-2
pubmed:meshHeading
pubmed-meshheading:12511180-Analysis of Variance, pubmed-meshheading:12511180-Animals, pubmed-meshheading:12511180-Animals, Genetically Modified, pubmed-meshheading:12511180-Citrulline, pubmed-meshheading:12511180-Denervation, pubmed-meshheading:12511180-Enzyme Inhibitors, pubmed-meshheading:12511180-Gene Expression Regulation, pubmed-meshheading:12511180-Hypertension, pubmed-meshheading:12511180-Kidney, pubmed-meshheading:12511180-Male, pubmed-meshheading:12511180-Matched-Pair Analysis, pubmed-meshheading:12511180-Nitric Oxide Synthase, pubmed-meshheading:12511180-Nitric Oxide Synthase Type I, pubmed-meshheading:12511180-RNA, Messenger, pubmed-meshheading:12511180-Rats, pubmed-meshheading:12511180-Rats, Inbred Strains, pubmed-meshheading:12511180-Renal Circulation, pubmed-meshheading:12511180-Renin, pubmed-meshheading:12511180-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:12511180-Sympathetic Nervous System, pubmed-meshheading:12511180-Thiourea
pubmed:year
2002
pubmed:articleTitle
Role of nNOS in regulation of renal function in hypertensive Ren-2 transgenic rats.
pubmed:affiliation
Department of Experimental Medicine, Institute for Clinical and Experimental Medicine, Prague, Czech Republic.luce@medicon.cz
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't