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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-12-31
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pubmed:abstractText |
Polymerase chain reaction (PCR) is a sensitive method for detection of Aspergillus DNA in bronchoalveolar lavage fluid, but it has not yet been able to distinguish infection from contamination. We have established a technique to quantify Aspergillus DNA using a real-time PCR method to resolve this problem, and we report herein a successful application of real-time PCR to diagnose invasive pulmonary aspergillosis by comparing the amount of Aspergillus DNA in bronchial lavage fluid from an affected area to that from an unaffected area. This novel tool will provide rapid, sensitive, and specific diagnosis of pulmonary aspergillosis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0361-8609
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pubmed:author |
pubmed-author:AokiKatsunoriK,
pubmed-author:ChibaShigeruS,
pubmed-author:GoyamaSusumuS,
pubmed-author:HiraiHisamaruH,
pubmed-author:KandaYoshinobuY,
pubmed-author:KawazuMasahitoM,
pubmed-author:KomenoYukikoY,
pubmed-author:KurokawaMineoM,
pubmed-author:MotokuraToruT,
pubmed-author:NakamotoTetsuyaT,
pubmed-author:NannyaYasuhitoY,
pubmed-author:NiinoMiyukiM,
pubmed-author:OgawaSeishiS,
pubmed-author:OhishiNobuyaN,
pubmed-author:TakeshitaMasatakaM,
pubmed-author:TsujinoShihoS
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pubmed:copyrightInfo |
Copyright 2002 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
72
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
27-30
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12508264-Adult,
pubmed-meshheading:12508264-Aspergillosis,
pubmed-meshheading:12508264-Aspergillus fumigatus,
pubmed-meshheading:12508264-Bone Marrow Transplantation,
pubmed-meshheading:12508264-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:12508264-Computer Systems,
pubmed-meshheading:12508264-Cryptogenic Organizing Pneumonia,
pubmed-meshheading:12508264-DNA, Fungal,
pubmed-meshheading:12508264-Female,
pubmed-meshheading:12508264-Graft vs Host Disease,
pubmed-meshheading:12508264-Humans,
pubmed-meshheading:12508264-Immunosuppressive Agents,
pubmed-meshheading:12508264-Leukemia, Myeloid, Acute,
pubmed-meshheading:12508264-Lung Diseases, Fungal,
pubmed-meshheading:12508264-Opportunistic Infections,
pubmed-meshheading:12508264-Polymerase Chain Reaction,
pubmed-meshheading:12508264-Sensitivity and Specificity,
pubmed-meshheading:12508264-Transplantation, Homologous
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pubmed:year |
2003
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pubmed:articleTitle |
Rapid diagnosis of invasive pulmonary aspergillosis by quantitative polymerase chain reaction using bronchial lavage fluid.
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pubmed:affiliation |
Department of Hematology & Oncology, University of Tokyo, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Case Reports,
Evaluation Studies
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