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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2002-12-30
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pubmed:abstractText |
We have previously reported that high glucose stimulates osteopontin (OPN) expression via a protein kinase C-dependent pathway and a hexosamine pathway in cultured rat aortic smooth muscle cells (SMCs) [Biochem. Biophys. Res. Commun. 258 (1999) 722.]. In the present study, we carried out functional OPN promoter assays using the luciferase expression vector system in cultured rat aortic SMCs to determine a high glucose/glucosamine responsive element. An extensive deletion analysis of the 5'-flanking region of the rat OPN gene revealed that an element involved in high glucose and glucosamine responses was present within a region between -112 and -62 bp of the OPN promoter. This region is highly conserved in the rat, mouse, and human promoters and contains a number of consensus regions, including an E-box and a GC-rich region. Mutation of the E-box or the GC-rich region resulted in a significant loss of both high glucose and glucosamine responses. These results suggest that two cis-acting elements, the E-box and the GC-rich region, are involved at least partly in high glucose/glucosamine-stimulated transcription of the rat OPN gene.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1056-8727
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pubmed:author |
pubmed-author:AsaumiSunaoS,
pubmed-author:ButlerWilliam TWT,
pubmed-author:FujimotoMasakiM,
pubmed-author:KawamuraHarukiyoH,
pubmed-author:KobayashiKazukiK,
pubmed-author:MoriSeijiroS,
pubmed-author:RidallAmy LAL,
pubmed-author:SaitoYasushiY,
pubmed-author:TakeAyakoA,
pubmed-author:TakemotoMinoruM,
pubmed-author:YokoteKoutaroK
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pubmed:issnType |
Print
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
34-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:12505755-Animals,
pubmed-meshheading:12505755-Aorta,
pubmed-meshheading:12505755-Base Sequence,
pubmed-meshheading:12505755-Cells, Cultured,
pubmed-meshheading:12505755-Conserved Sequence,
pubmed-meshheading:12505755-Gene Deletion,
pubmed-meshheading:12505755-Gene Expression,
pubmed-meshheading:12505755-Glucosamine,
pubmed-meshheading:12505755-Glucose,
pubmed-meshheading:12505755-Humans,
pubmed-meshheading:12505755-Male,
pubmed-meshheading:12505755-Mice,
pubmed-meshheading:12505755-Muscle, Smooth, Vascular,
pubmed-meshheading:12505755-Mutagenesis,
pubmed-meshheading:12505755-Osteopontin,
pubmed-meshheading:12505755-Promoter Regions, Genetic,
pubmed-meshheading:12505755-Rats,
pubmed-meshheading:12505755-Rats, Wistar,
pubmed-meshheading:12505755-Response Elements,
pubmed-meshheading:12505755-Sialoglycoproteins,
pubmed-meshheading:12505755-Transcription, Genetic,
pubmed-meshheading:12505755-Transfection
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pubmed:articleTitle |
Identification and characterization of high glucose and glucosamine responsive element in the rat osteopontin promoter.
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pubmed:affiliation |
Department of Clinical Cell Biology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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