Source:http://linkedlifedata.com/resource/pubmed/id/12504570
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rdf:type | |
lifeskim:mentions |
umls-concept:C0028326,
umls-concept:C0029347,
umls-concept:C0029348,
umls-concept:C0033618,
umls-concept:C0033684,
umls-concept:C0305052,
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umls-concept:C0678594,
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umls-concept:C1522138,
umls-concept:C1527178,
umls-concept:C1706044,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
2
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pubmed:dateCreated |
2002-12-30
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pubmed:abstractText |
The N-terminal domains of the NS1 protein of influenza B virus (NS1B protein) and the NS1 protein of influenza A virus (NS1A protein) share one function: binding double-stranded RNA (dsRNA). Here we show that the N-terminal domain of the NS1B protein possesses an additional function that is not shared by its NS1A counterpart: binding the ubiquitin-like ISG15 protein that is induced by influenza B virus infection. Homology modeling predicts that the dimeric six-helical N-terminal domain of the NS1B protein differs from its NS1A protein counterpart in containing large loops between helices 1 and 2 (loops 1 and 1') and between helices 2 and 3 (loops 2 and 2'). Mutagenesis establishes that residues located in loop 1/1' together with residues located in polypeptide segment 94-103 form the ISG15 protein-binding site of NS1B protein. Loop 1/1' is not required for dsRNA binding, which instead requires arginine residues R50, R53, R50', and R53' located in antiparallel helices 1 and 1'. Further, we demonstrate that the binding sites for RNA and protein are independent of each other. In particular, ISG15 and dsRNA can bind simultaneously; the binding of the ISG15 protein does not have a detectable effect on the binding of dsRNA, and vice versa.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/INS1 protein, influenza virus,
http://linkedlifedata.com/resource/pubmed/chemical/ISG15 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Ubiquitins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Nonstructural Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0042-6822
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 2002 Elsevier Science (USA)
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pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
304
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
291-301
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pubmed:dateRevised |
2008-5-6
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pubmed:meshHeading |
pubmed-meshheading:12504570-Amino Acid Sequence,
pubmed-meshheading:12504570-Binding Sites,
pubmed-meshheading:12504570-Cytokines,
pubmed-meshheading:12504570-Dimerization,
pubmed-meshheading:12504570-Influenza B virus,
pubmed-meshheading:12504570-Models, Molecular,
pubmed-meshheading:12504570-Molecular Sequence Data,
pubmed-meshheading:12504570-Protein Binding,
pubmed-meshheading:12504570-RNA, Viral,
pubmed-meshheading:12504570-Ubiquitins,
pubmed-meshheading:12504570-Viral Nonstructural Proteins
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pubmed:year |
2002
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pubmed:articleTitle |
Structural basis for ubiquitin-like ISG 15 protein binding to the NS1 protein of influenza B virus: a protein-protein interaction function that is not shared by the corresponding N-terminal domain of the NS1 protein of influenza A virus.
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pubmed:affiliation |
Institute for Cellular and Molecular Biology, Section of Molecular Genetics and Microbiology, University of Texas at Austin, 78712, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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