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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2002-12-30
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pubmed:abstractText |
A novel fibrinogenolytic protease, named alpha-mucrofibrase, was purified from the venom of Chinese Habu (Trimeresurus mucrosquamatus) by DEAE-Sephadex A-50 ion-exchange chromatography and Sephadex G-100 (super fine) gel filtration alpha-Mucrofibrase is a single-chain polypeptide of approximately 29 kDa. It is stable even at 95 degrees C, and the most susceptible hydrolysis substrate is S-2302. It cleaved primarily the Aalpha chain of fibrinogen followed by the Bbeta chain, while the gamma chain was partially affected. N-terminal sequence of this fibrinogenolytic enzyme has great homology with those of other snake venom serine proteases. The esterase activity of alpha-mucrofibrase is inhibited by phenylmethylsulfonyl fluoride (PMSF) but not by metal chelator (EDTA), suggesting this fibrinogenase belongs to the venom serine protease family.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1058-8108
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
337-43
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:12503877-Amino Acid Sequence,
pubmed-meshheading:12503877-Animals,
pubmed-meshheading:12503877-Chromatography, DEAE-Cellulose,
pubmed-meshheading:12503877-Chromatography, Ion Exchange,
pubmed-meshheading:12503877-Crotalid Venoms,
pubmed-meshheading:12503877-Enzyme Stability,
pubmed-meshheading:12503877-Fibrinogen,
pubmed-meshheading:12503877-Hemorrhage,
pubmed-meshheading:12503877-Metalloendopeptidases,
pubmed-meshheading:12503877-Mice,
pubmed-meshheading:12503877-Molecular Sequence Data,
pubmed-meshheading:12503877-Platelet Aggregation,
pubmed-meshheading:12503877-Rabbits,
pubmed-meshheading:12503877-Sequence Homology, Amino Acid,
pubmed-meshheading:12503877-Serine Endopeptidases,
pubmed-meshheading:12503877-Serine Proteinase Inhibitors,
pubmed-meshheading:12503877-Substrate Specificity,
pubmed-meshheading:12503877-Trimeresurus
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pubmed:year |
2002
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pubmed:articleTitle |
Purification and characterization of alpha-mucrofibrase, a novel serine protease with alpha-fibrinogenase activity from the venom of Chinese Habu (Trimeresurus mucrosquamatus).
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pubmed:affiliation |
Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China.
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pubmed:publicationType |
Journal Article
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